Ho G, Campbell W H, Carlson E
Department of Biological Sciences, Michigan Technological University, Houghton 49931.
J Clin Microbiol. 1989 Jan;27(1):210-2. doi: 10.1128/jcm.27.1.210-212.1989.
A toxic shock syndrome toxin 1 (TSST-1) antibody-binding protein produced by an ovine-associated strain of Staphylococcus aureus was examined. The protein showed total identity to TSST-1 by immunodiffusion analysis. Western blots (immunoblots) of proteins separated by isoelectric focusing revealed that the TSST-1 antibody-binding protein had a pI of 8.6 rather than 7.0, the pI of standard TSST-1.
对一株与绵羊相关的金黄色葡萄球菌产生的一种毒性休克综合征毒素1(TSST-1)抗体结合蛋白进行了检测。通过免疫扩散分析,该蛋白与TSST-1完全一致。对经等电聚焦分离的蛋白质进行的蛋白质印迹法(免疫印迹法)显示,TSST-1抗体结合蛋白的等电点为8.6,而标准TSST-1的等电点为7.0。