Kruger T E, Smith L R, Harbour D V, Blalock J E
Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
J Immunol. 1989 Feb 1;142(3):744-7.
We have previously shown that thyrotropin (TSH), which is produced by lymphocytes in response to the T cell mitogen staphylococcal enterotoxin A, enhances in vitro antibody production to T cell-dependent and independent Ag (SRBC and trinitrophenylated Brucella abortus [BA-TPN], respectively) as determined by a direct plaque-forming cell assay. As a result of these studies, experiments were designed to examine the possible immunoregulatory function of thyrotropin-releasing hormone (TRH) on the in vitro antibody response to the T cell-independent Ag BA-TNP. Our studies demonstrate that TRH at very low concentrations (pM) enhances the in vitro plaque-forming cell response to BA-TNP and also induces splenocyte production of TSH. Other hypothalamic-releasing factors were without effect. This enhancement effect by TRH was specifically blocked by rabbit antisera to the TSH-beta subunit, whereas addition of normal rabbit sera had no effect. These data suggest that TRH specifically enhances the in vitro antibody response via production of immunoreactive TSH.
我们之前已经表明，淋巴细胞在T细胞丝裂原葡萄球菌肠毒素A的刺激下产生的促甲状腺激素（TSH），通过直接空斑形成细胞试验测定，可增强体外对T细胞依赖性和非依赖性抗原（分别为绵羊红细胞和三硝基苯基化流产布鲁氏菌[BA-TPN]）的抗体产生。基于这些研究结果，设计了实验来检测促甲状腺激素释放激素（TRH）对体外针对T细胞非依赖性抗原BA-TNP的抗体反应可能具有的免疫调节功能。我们的研究表明，极低浓度（皮摩尔）的TRH可增强体外对BA-TNP的空斑形成细胞反应，还可诱导脾细胞产生TSH。其他下丘脑释放因子则无此作用。TRH的这种增强作用被针对TSH-β亚基的兔抗血清特异性阻断，而添加正常兔血清则无影响。这些数据表明，TRH通过产生免疫反应性TSH特异性增强体外抗体反应。
