Fuursted K
Department of Clinical Microbiology, Odense University Hospital, Denmark.
APMIS. 1989 Jan;97(1):27-32. doi: 10.1111/j.1699-0463.1989.tb00750.x.
The influence of various incubation conditions on synergism with cell wall-active agents combined with streptomycin was studied in a Streptococcus faecalis strain. It could be shown that both a synergistic increased killing effect and a synergistic prolongation of post-antibiotic effect (PAE) were not dependent on an active process, as they occurred both in an anaerobic atmosphere and when an electron transport inhibitor (sodium azide) was added to the media. Moreover, it was demonstrated that if a drug, alone or in combination with streptomycin, had a PAE, there was an increased susceptibility to a subinhibitory concentration of streptomycin (1/4xMIC) when added to a culture recovering from its PAE. These findings confirm the commonly held belief in a mechanistic mechanism of synergism: That cell wall-active agents modify in some way the cell-envelope, thereby enhancing aminoglycoside uptake and killing in Streptococcus faecalis.
在一株粪肠球菌中研究了各种培养条件对细胞壁活性药物与链霉素联合使用时协同作用的影响。结果表明，协同增强的杀菌效果和协同延长的抗生素后效应（PAE）均不依赖于活性过程，因为它们在厌氧环境中以及向培养基中添加电子传递抑制剂（叠氮化钠）时均会出现。此外，研究还表明，如果一种药物单独或与链霉素联合使用具有PAE，那么当将其添加到从PAE恢复的培养物中时，对亚抑菌浓度的链霉素（1/4xMIC）的敏感性会增加。这些发现证实了人们普遍认为的协同作用机制：即细胞壁活性药物以某种方式修饰细胞包膜，从而增强粪肠球菌对氨基糖苷类药物的摄取和杀伤作用。
