Fuursted K
Department of Clinical Microbiology, Odense University Hospital, Denmark.
Chemotherapy. 1988;34(3):229-34. doi: 10.1159/000238574.
Nine strains of Streptococcus faecalis and Streptococcus faecium were studied with respect to ampicillin, ciprofloxacin, imipenem, piperacillin, vancomycin and streptomycin. Two strains were highly resistant (MIC greater than or equal to 2,000 micrograms/ml) to streptomycin. Evaluation and comparison of the killing activity with killing curves, and duration of the postantibiotic effect (PAE) after exposure for 1 h with regrowth curves was done with combinations of antibiotics or alone. The overall killing effect of ciprofloxacin with streptomycin was antagonistic, whereas synergism (greater than or equal to one log10 decrease in viable counts) was observed in streptomycin-susceptible strains with combinations of streptomycin and ampicillin, imipenem, piperacillin or vancomycin. In addition, prolongation of PAE (greater than or equal to 0.5 h) was seen only in streptomycin-susceptible strains. Thus, seven (100%) strains showed a synergistic increase in PAE to combinations with ampicillin and vancomycin, three (43%) to imipenem, four (57%) to piperacillin, but none to the combination of streptomycin and ciprofloxacin. A significant correlation was observed between the magnitude of increased killing and the extent of increase in recovery period with combinations of streptomycin with either ampicillin or vancomycin.
对9株粪肠球菌和屎肠球菌进行了氨苄西林、环丙沙星、亚胺培南、哌拉西林、万古霉素和链霉素方面的研究。有两株菌对链霉素高度耐药（最低抑菌浓度大于或等于2000微克/毫升）。采用抗生素联合用药或单独用药，通过杀菌曲线评估和比较杀菌活性，并通过再生长曲线对暴露1小时后的抗生素后效应（PAE）持续时间进行了研究。环丙沙星与链霉素的总体杀菌效果呈拮抗作用，而在链霉素敏感菌株中，链霉素与氨苄西林、亚胺培南、哌拉西林或万古霉素联合使用时观察到协同作用（活菌数减少大于或等于1个对数10）。此外，仅在链霉素敏感菌株中观察到PAE延长（大于或等于0.5小时）。因此，7株（100%）菌株对氨苄西林和万古霉素联合用药的PAE呈协同增加，3株（43%）对亚胺培南，4株（57%）对哌拉西林，但对链霉素和环丙沙星联合用药无此现象。在链霉素与氨苄西林或万古霉素联合使用时，杀菌增加幅度与恢复期延长程度之间存在显著相关性。
