Albertoni Guilherme, Castelo Girão Manoel João Batista, Schor Nestor
Federal University of São Paulo (UNIFESP), Department of Medicine, Nephrology Division, Rua Botucatu 740, São Paulo, 04023-900, SP, Brazil; Colsan (Associação Beneficente de Coleta de Sangue), São Paulo, SP, Brazil.
Colsan (Associação Beneficente de Coleta de Sangue), São Paulo, SP, Brazil.
Int J Infect Dis. 2014 Aug;25:145-9. doi: 10.1016/j.ijid.2014.04.007. Epub 2014 Jun 10.
The detection of acute human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) infection is vital for controlling the spread of HIV, HBV, and HCV to uninfected individuals. Considering that these viruses have high replication rates and are undetectable by serological markers, early detection upon transmission is crucial. Various nucleic acid assays have been developed for diagnostics and therapeutic monitoring of infections. In the past decade, rapid and sensitive molecular techniques such as PCR have revolutionized the detection of a variety of infectious viruses, including HIV, HCV, and HBV. Here, we describe two of the most commonly used licensed methods for the detection and quantification of HIV, HCV, and HBV: the cobas TaqScreen MPX (PCR) test and the Tigris System. We used transcription-mediated amplification to review and compare the development and efficiency of these technologies.
急性人类免疫缺陷病毒（HIV）、乙型肝炎病毒（HBV）和丙型肝炎病毒（HCV）感染的检测对于控制HIV、HBV和HCV向未感染个体的传播至关重要。鉴于这些病毒具有高复制率且血清学标志物无法检测到，传播时的早期检测至关重要。已开发出各种核酸检测方法用于感染的诊断和治疗监测。在过去十年中，诸如PCR等快速且灵敏的分子技术彻底改变了包括HIV、HCV和HBV在内的多种传染性病毒的检测。在此，我们描述两种最常用的已获许可的用于检测和定量HIV、HCV和HBV的方法：cobas TaqScreen MPX（PCR）检测和Tigris系统。我们使用转录介导扩增来回顾和比较这些技术的发展及效率。
