Kharaziha Mahshid, Shin Su Ryon, Nikkhah Mehdi, Topkaya Seda Nur, Masoumi Nafiseh, Annabi Nasim, Dokmeci Mehmet R, Khademhosseini Ali
Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Woman's Hospital, Harvard Medical School, Boston, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, 8415683111, Iran.
Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Woman's Hospital, Harvard Medical School, Boston, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
Biomaterials. 2014 Aug;35(26):7346-54. doi: 10.1016/j.biomaterials.2014.05.014. Epub 2014 Jun 10.
In the past few years, a considerable amount of effort has been devoted toward the development of biomimetic scaffolds for cardiac tissue engineering. However, most of the previous scaffolds have been electrically insulating or lacked the structural and mechanical robustness to engineer cardiac tissue constructs with suitable electrophysiological functions. Here, we developed tough and flexible hybrid scaffolds with enhanced electrical properties composed of carbon nanotubes (CNTs) embedded aligned poly(glycerol sebacate):gelatin (PG) electrospun nanofibers. Incorporation of varying concentrations of CNTs from 0 to 1.5% within the PG nanofibrous scaffolds (CNT-PG scaffolds) notably enhanced fiber alignment and improved the electrical conductivity and toughness of the scaffolds while maintaining the viability, retention, alignment, and contractile activities of cardiomyocytes (CMs) seeded on the scaffolds. The resulting CNT-PG scaffolds resulted in stronger spontaneous and synchronous beating behavior (3.5-fold lower excitation threshold and 2.8-fold higher maximum capture rate) compared to those cultured on PG scaffold. Overall, our findings demonstrated that aligned CNT-PG scaffold exhibited superior mechanical properties with enhanced CM beating properties. It is envisioned that the proposed hybrid scaffolds can be useful for generating cardiac tissue constructs with improved organization and maturation.
在过去几年中，人们投入了大量精力用于开发用于心脏组织工程的仿生支架。然而，以前的大多数支架都是电绝缘的，或者缺乏构建具有合适电生理功能的心脏组织构建体所需的结构和机械强度。在此，我们开发了一种坚韧且灵活的混合支架，其具有增强的电学性能，该支架由嵌入排列的聚（癸二酸甘油酯）：明胶（PG）电纺纳米纤维中的碳纳米管（CNT）组成。在PG纳米纤维支架（CNT-PG支架）中加入0至1.5%不同浓度的CNT，显著增强了纤维排列，提高了支架的导电性和韧性，同时保持了接种在支架上的心肌细胞（CM）的活力、留存率、排列和收缩活性。与在PG支架上培养的细胞相比，所得的CNT-PG支架产生了更强的自发和同步跳动行为（兴奋阈值低3.5倍，最大捕获率高2.8倍）。总体而言，我们的研究结果表明，排列的CNT-PG支架具有优异的机械性能，同时增强了CM跳动性能。可以设想，所提出的混合支架可用于生成具有更好组织和成熟度的心脏组织构建体。
