通过聚多巴胺涂层将胶原蛋白共价固定在钛上以改善MC3T3-E1细胞的细胞性能
Covalent Immobilization of Collagen on Titanium through Polydopamine Coating to Improve Cellular Performances of MC3T3-E1 Cells.
作者信息
Yu Xiaohua, Walsh John, Wei Mei
机构信息
Department of Materials Science and Engineering, University of Connecticut Storrs, CT, 06269, USA.
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA.
出版信息
RSC Adv. 2013 Nov 21;4(14):7185-7192. doi: 10.1039/C3RA44137G.
Surface modification of orthopedic implants is critical for improving the clinical performance of these medical devices. Herein, collagen was covalently immobilized onto a titanium implant surface via a novel adherent polydopamine coating inspired by mussel adhesive proteins. The formation and composition of the collagen coating was characterized using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Fluorescent labeled collagen was also used to examine the formation and uniformity of the collagen coating. The resultant collagen coating with a polydopamine supporting substrate demonstrated better uniformity and distribution on the titanium surface compared to a physical adsorption of collagen. The covalent immobilized collagen coating is biologically active, as evidenced by its ability to enhance MC3T3-E1 cell adhesion, support cell proliferation and promote early stage osteogenic differentiation of pre-osteoblasts. Our study suggests covalent immobilization of collagen through the polydopamine coating might be an efficient way to improve the cellular performance of implant surfaces.
骨科植入物的表面改性对于改善这些医疗器械的临床性能至关重要。在此，受贻贝粘附蛋白启发，通过一种新型的粘附性聚多巴胺涂层将胶原蛋白共价固定在钛植入物表面。使用X射线光电子能谱（XPS）和扫描电子显微镜（SEM）对胶原蛋白涂层的形成和组成进行了表征。还使用荧光标记的胶原蛋白来检查胶原蛋白涂层的形成和均匀性。与胶原蛋白的物理吸附相比，具有聚多巴胺支撑基质的所得胶原蛋白涂层在钛表面上表现出更好的均匀性和分布。共价固定的胶原蛋白涂层具有生物活性，这通过其增强MC3T3-E1细胞粘附、支持细胞增殖和促进前成骨细胞早期成骨分化的能力得到证明。我们的研究表明，通过聚多巴胺涂层共价固定胶原蛋白可能是改善植入物表面细胞性能的有效方法。
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