Alurappa Ramesha, Bojegowda Madhusudhan Reddy Muthukurpalya, Kumar Vijith, Mallesh Naveen Kumar, Chowdappa Srinivas
a Department of Microbiology and Biotechnology , Bangalore University , Jnanabharathi Campus, Bangalore , Karnataka 560 056 , India.
Nat Prod Res. 2014;28(23):2217-20. doi: 10.1080/14786419.2014.924933. Epub 2014 Jun 17.
Bioactive compounds comprising secondary metabolites produced by endophytic fungi have wide applications in pharmacology and agriculture. Isolation, characterisation and evaluation of biological activities of secondary metabolites were carried out from Cochliobolus kusanoi an endophytic fungus of Nerium oleander L. The fungus was identified based on 18S rDNA sequence analysis. There are no reports available on the compounds of C.kusanoi hence, antimicrobial metabolite produced by this fungus was extracted and purified by fractionation using hexane, diethyl ether, dichloromethane, ethyl acetate and methanol. Out of all the solvent fractions, the methanol fraction exhibited better antimicrobial activity which was further purified and characterised as oosporein. Oosporein from C.kusanoi exhibited broad spectrum in vitro antimicrobial, antioxidant and cytotoxic activities. The characterisation and antioxidant activity of oosporein from C. kusanoi are reported for the first time.
由内生真菌产生的包含次生代谢产物的生物活性化合物在药理学和农业领域有广泛应用。从夹竹桃内生真菌库氏旋孢腔菌中进行次生代谢产物的分离、表征及生物活性评估。该真菌基于18S rDNA序列分析进行鉴定。目前尚无关于库氏旋孢腔菌化合物的报道，因此，通过用己烷、乙醚、二氯甲烷、乙酸乙酯和甲醇进行分馏，提取并纯化了该真菌产生的抗菌代谢产物。在所有溶剂馏分中，甲醇馏分表现出更好的抗菌活性，进一步纯化并鉴定为卵孢素。库氏旋孢腔菌的卵孢素在体外具有广谱抗菌、抗氧化和细胞毒性活性。首次报道了库氏旋孢腔菌卵孢素的表征及抗氧化活性。
