Yakult Central Institute for Microbiological Research, Kunitachi 186-8650, Japan.
Faculty of Research and Development, Yakult Honsha, Shimbashi, Tokyo 105-8660, Japan.
Biosci Microbiota Food Health. 2013;32(1):33-9. doi: 10.12938/bmfh.32.33. Epub 2013 Jan 31.
A double-blind, placebo-controlled, randomized trial was conducted to investigate the beneficial effect of probiotic and prebiotic fermented milk on the skin of healthy adult women. Forty healthy Japanese adult female volunteers with healthy skin randomly received either a bottle of probiotic and prebiotic fermented milk containing Bifidobacterium breve strain Yakult and galacto-oligosaccharides (GOS) (active group) or a non-fermented placebo milk containing neither probiotics nor GOS (placebo group) daily for 4 weeks. Before and after intake, hydration levels and cathepsin L-like activity in the stratum corneum and phenol levels in the serum and urine were determined. After intake, the hydration level of the stratum corneum decreased significantly in only the placebo group and was significantly lower than in the active group (p=0.031). Cathepsin L-like activity, an indicator of keratinocyte differentiation, was significantly increased in the active group (p=0.027). Serum and urine phenol levels decreased significantly in the active group (p=0.014, p=0.002, respectively), and serum phenol levels were significantly lower in the active group compared with the placebo group (p=0.006). The consecutive intake of probiotic and prebiotic fermented milk can benefit skin condition without dryness and decrease the levels of phenol production by gut bacteria in healthy adult women.
一项双盲、安慰剂对照、随机试验研究了益生菌和益生元发酵乳对健康成年女性皮肤的有益作用。40 名皮肤健康的日本成年女性志愿者随机接受含有短双歧杆菌 Yakult 和半乳糖寡糖（GOS）的益生菌和益生元发酵乳（实验组）或既不含益生菌也不含 GOS 的非发酵安慰剂牛奶（对照组），每天一次，连续 4 周。在摄入前后，测定角质层的水分含量和组织蛋白酶 L 样活性以及血清和尿液中的苯酚水平。摄入后，只有安慰剂组的角质层水分含量显著下降，且明显低于实验组（p=0.031）。角质形成细胞分化的指标组织蛋白酶 L 样活性在实验组显著增加（p=0.027）。实验组血清和尿液中的苯酚水平显著下降（p=0.014，p=0.002），且实验组血清苯酚水平明显低于安慰剂组（p=0.006）。连续摄入益生菌和益生元发酵乳可以改善皮肤状况，不会使皮肤干燥，并减少健康成年女性肠道细菌产生的苯酚水平。
