Mannas Hasna, Mimouni Rachida, Chaouqy Noureddine, Hamadi Fatima, Martinez-Urtaza Jaime
Faculty of Sciences, Laboratory of Biotechnology & Valorisation of Natural Resources, University Ibn Zohr, Agadir, Morocco.
Laboratory of Microbiology, National Health Security Food Office (ONSSA), Agadir, Morocco.
Springerplus. 2014 May 24;3:265. doi: 10.1186/2193-1801-3-265. eCollection 2014.
This study reports the occurrence of different Vibrio and Salmonella species in 52 samples of Mytilus galloprovincialis collected from four sites along the Atlantic coast between Agadir and Essaouira (Anza, Cap Ghir, Imssouane and Essaouira). The level of Escherichia coli (E. coli) was also determined to evaluate the degree of microbial pollution in the investigated areas. In this study three methods were used : AFNOR NF EN ISO 6579 V08-013 for Salmonella spp., the provisional method routinely used by several laboratories (Institut Pasteur, Paris,…) for Vibrio cholerae and Vibrio parahaemolyticus in the seafood, and the most probable number method (MPN) using Norm ISO/TS 16649-3 (2005) for E. coli. The most frequently isolated Vibrios were Vibrio alginolyticus (90.4% of samples), followed by V. cholerae non O1 non O139 (15.4%) and V. parahaemolyticus (7.7%). Salmonella spp. was found in 15% of the samples. The number of E. coli ranged between 0.2/100 g and 1.8 10(3) /100 g of mussel soft tissues. This study indicates the potential sanitary risk associated with the presence of pathogenic bacteria in cultivated mussels in the two populous regions of southern Morocco, where shellfish production and maritime tourism are important to the local economy.
本研究报告了从阿加迪尔和索维拉之间大西洋沿岸的四个地点（安扎、吉尔角、伊姆苏阿内和索维拉）采集的52份加利福尼亚贻贝样本中不同弧菌属和沙门氏菌属的出现情况。还测定了大肠杆菌（E. coli）水平，以评估调查区域的微生物污染程度。在本研究中使用了三种方法：用于沙门氏菌属的法国标准化协会标准NF EN ISO 6579 V08 - 013、几个实验室（巴黎巴斯德研究所等）常规使用的用于海鲜中霍乱弧菌和副溶血性弧菌的临时方法，以及使用标准ISO/TS 16649 - 3（2005）的大肠杆菌最可能数法（MPN）。最常分离出的弧菌是溶藻弧菌（占样本的90.4%），其次是非O1非O139群霍乱弧菌（15.4%）和副溶血性弧菌（7.7%）。在15%的样本中发现了沙门氏菌属。每100克贻贝软组织中的大肠杆菌数量在0.2至1.8×10³ 之间。本研究表明，在摩洛哥南部两个人口众多的地区，养殖贻贝中存在致病细菌会带来潜在的卫生风险，在这些地区贝类生产和海洋旅游对当地经济很重要。
