使用ProteoWizard转换原始质谱数据。
Employing ProteoWizard to Convert Raw Mass Spectrometry Data.
作者信息
Holman Jerry D, Tabb David L, Mallick Parag
机构信息
Department of Biomedical Informatics, Vanderbilt University School of Medicine, Nashville, Tennessee.
Department of Radiology, Stanford School of Medicine, Palo Alto, California.
出版信息
Curr Protoc Bioinformatics. 2014 Jun 17;46:13.24.1-13.24.9. doi: 10.1002/0471250953.bi1324s46.
Abstract
After raw data have been captured by mass spectrometers in biological LC-MS/MS experiments, they must be converted from vendor-specific binary files to open-format files for manipulation by most software. This protocol details the use of ProteoWizard software for this conversion, taking format features, coding options, and vendor particularities into account. This protocol will aid researchers in preparing their data for analysis by database search engines and other bioinformatics tools.
摘要
在生物液相色谱-串联质谱（LC-MS/MS）实验中，质谱仪捕获原始数据后，必须将其从特定供应商的二进制文件转换为开放格式文件，以便大多数软件进行处理。本方案详细介绍了使用ProteoWizard软件进行这种转换，同时考虑了格式特征、编码选项和供应商的特殊性。本方案将帮助研究人员为使用数据库搜索引擎和其他生物信息学工具进行分析准备数据。
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