使用ProteoWizard msConvert进行数据转换。

Data Conversion with ProteoWizard msConvert.

作者信息

Adusumilli Ravali, Mallick Parag

机构信息

Canary Center at Stanford for Cancer Early Detection, Department of Radiology, Stanford University, Stanford, CA, USA.

School of Medicine, Stanford University, 3155 Porter Drive, MC 5483, Palo Alto, CA, 94304, USA.

出版信息

Methods Mol Biol. 2017;1550:339-368. doi: 10.1007/978-1-4939-6747-6_23.

DOI:10.1007/978-1-4939-6747-6_23

PMID:28188540

Abstract

Recent advances in proteome informatics have led to an explosion in tools to analyze mass spectrometry data. These tools operate across the analysis pipeline doing everything from assessing quality control to matching peptides to spectra to quantification. Unfortunately, the vast majority of these tools are not able to operate directly on the proprietary formats generated by the diverse mass spectrometers. Consequently, the first step in many protocols is the conversion of data from vendor-specific binary files to open-format files. This protocol details the use of ProteoWizard's msConvert and msConvertGUI software for this conversion, taking format features, coding options, and vendor particularities into account. We specifically describe the various options available when doing conversions and the implications of each option.

摘要

蛋白质组信息学的最新进展导致了用于分析质谱数据的工具呈爆炸式增长。这些工具贯穿整个分析流程，执行从评估质量控制到将肽段与光谱匹配再到定量的所有操作。不幸的是，这些工具中的绝大多数无法直接处理由各种质谱仪生成的专有格式。因此，许多实验方案的第一步是将数据从特定供应商的二进制文件转换为开放格式文件。本实验方案详细介绍了使用ProteoWizard的msConvert和msConvertGUI软件进行这种转换，同时考虑了格式特征、编码选项和供应商特性。我们具体描述了进行转换时可用的各种选项以及每个选项的影响。