Cheuk Isabella Wai Yin, Yip Shea Ping, Kwong Dora Lai Wan, Wu Vincent Wing Cheung
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, SAR, P.R. China.
Department of Clinical Oncology, Queen Mary Hospital, The University of Hong Kong, Pok Fu Lam, Hong Kong, SAR, P.R. China.
Mol Clin Oncol. 2014 Jul;2(4):553-558. doi: 10.3892/mco.2014.276. Epub 2014 Apr 14.
Radiation-induced fibrosis is one of the late complications of radiotherapy (RT) for nasopharyngeal carcinoma (NPC). The aim of this study was to investigate the association between X-ray repair cross-complementing protein 1 and 3 ( and , respectively) gene haplotypes and radiation-induced fibrosis in NPC patients. Genomic DNA was extracted from blood samples of 120 NPC patients previously treated with RT. In total, 12 tag single-nucleotide polymorphisms (SNPs) were selected from the and genes and were genotyped using restriction fragment length polymorphism analysis or unlabeled probe melting analysis. Single-marker and haplotype analyses were performed using multivariate logistic regression analysis. The functional variant rs861539 of may be associated with radiation-induced fibrosis [asymptotic P-value (P)<0.05]. No significant association was observed between radiation-induced fibrosis and any of the tag SNPs of and in either single-marker or haplotype analysis after 10,000 permutations [empirical P-value (P)>0.05]. Our preliminary results indicated that the rs861539 variant of may be associated with an increased risk of radiation-induced fibrosis; however, a large-scale study is required to confirm this result.
放射性纤维化是鼻咽癌（NPC）放射治疗（RT）的晚期并发症之一。本研究旨在探讨X射线修复交叉互补蛋白1和3（分别为 和 ）基因单倍型与NPC患者放射性纤维化之间的关联。从120例先前接受过放疗的NPC患者的血液样本中提取基因组DNA。总共从 和 基因中选择了12个标签单核苷酸多态性（SNP），并使用限制性片段长度多态性分析或未标记探针熔解分析进行基因分型。使用多变量逻辑回归分析进行单标记和单倍型分析。 的功能性变异rs861539可能与放射性纤维化相关[渐近P值（P）<0.05]。在10,000次置换后，无论是单标记分析还是单倍型分析，均未观察到放射性纤维化与 和 的任何标签SNP之间存在显著关联[经验P值（P）>0.05]。我们的初步结果表明， 的rs861539变异可能与放射性纤维化风险增加有关；然而，需要大规模研究来证实这一结果。
