Velasco Mary Grace M, Levene Michael J
Department of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA.
Biomed Opt Express. 2014 Apr 30;5(6):1700-8. doi: 10.1364/BOE.5.001700. eCollection 2014 Jun 1.
Two-photon microscopy has been used in conjunction with micro-optics, such as GRIN lenses, to access subcortical structures in the intact mouse brain. In this study, we demonstrate the use of thick glass windows, or plugs, for high-resolution, large field-of-view two-photon imaging of the hippocampus in a live mouse. These plugs are less expensive, yield larger fields-of-view and are simpler to use than GRIN lenses while requiring less tissue removal compared to previous methods based on cortical ablation. To demonstrate the capabilities of our system, we show fluorescence images of dendritic spines in the CA1 region of the hippocampus in THY1-YFP transgenic mice.
双光子显微镜已与微光学器件（如梯度折射率透镜）结合使用，以观察完整小鼠大脑中的皮层下结构。在本研究中，我们展示了使用厚玻璃窗或塞子对活小鼠海马体进行高分辨率、大视野双光子成像。这些塞子比梯度折射率透镜成本更低，视野更大，使用更简单，与基于皮层切除的先前方法相比，所需的组织切除更少。为了展示我们系统的能力，我们展示了THY1-YFP转基因小鼠海马体CA1区树突棘的荧光图像。
