Qin Zhongya, Chen Congping, He Sicong, Wang Ye, Tam Kam Fai, Ip Nancy Y, Qu Jianan Y
Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P. R. China.
State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P. R. China.
Sci Adv. 2020 Sep 30;6(40). doi: 10.1126/sciadv.abc6521. Print 2020 Sep.
Optical deep-brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep-brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.
几十年来，高分辨率的活体光学深部脑成像一直是一项巨大的挑战。双光子内镜检查提供了一种微创方法来对埋藏的脑结构进行成像，一旦它与嵌入大脑的梯度折射率（GRIN）透镜集成。然而，其成像分辨率和视野会受到GRIN透镜固有像差的影响。在这里，我们通过添加基于直接波前传感的自适应光学技术来开发一种双光子内镜检查，这使得在深部脑成像中能够恢复衍射极限分辨率。一种新的预补偿策略在校正大体积像差和实现快速随机访问多平面成像方面起着关键作用。我们研究了海马体（一个关键的深部脑结构）中的神经元可塑性，并揭示了锥体神经元的体细胞和树突活动之间的关系。
