Nunes de Paiva Maria José, Menezes Helvécio Costa, de Lourdes Cardeal Zenilda
Departamento de Química, ICEx, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627-31270901 Belo Horizonte, MG, Brazil.
Analyst. 2014 Aug 7;139(15):3683-94. doi: 10.1039/c4an00583j.
Metabolome analysis involves the study of small molecules that are involved in the metabolic responses that occur through patho-physiological changes caused by genetic stimuli or chemical agents. Qualitative and quantitative metabolome analyses are used for the diagnosis of various diseases or chemical exposure. This article presents an overview of the different analytical methods available for performing the determination of the metabolome, including sampling, sample preparation and processing and interpretation of data. Critical comments are aimed at emphasizing the extraction methods as well as the biological samples used for metabolome analysis and data processing.
代谢组学分析涉及对小分子的研究，这些小分子参与了由遗传刺激或化学试剂引起的病理生理变化所导致的代谢反应。定性和定量代谢组学分析用于各种疾病的诊断或化学物质暴露的检测。本文概述了用于测定代谢组的不同分析方法，包括采样、样品制备与处理以及数据解读。批判性评论旨在强调代谢组分析和数据处理中所使用的提取方法以及生物样品。
