Zou Ziying, Liu Yuan, Zhu Bing, Zeng Ping
Department of Microbiology and Immunology, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China.
Department of Clinical Chemistry, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China.
Exp Ther Med. 2014 Jul;8(1):115-117. doi: 10.3892/etm.2014.1705. Epub 2014 May 12.
A growing number of nontuberculous mycobacteria infection cases, especially those caused by rapidly growing mycobacteria (RGM), have been reported in the past decade. Conventional methods for mycobacteria diagnosis are inefficient and easily lead to misdiagnosis. New detection methods, such as gene sequencing, have been reported but are not widely used. The aim of the present case report was to provide a quick and exact method of identifying infections. The particular case reported in this study initially manifested as hyperglycemia and papules in the right leg. Routine cultures for fungus were repeatedly negative. However, cultures of the purulent material under aerobic cultivation for five days yielded a rapidly growing, nontuberculous mycobacterium. A Ziehl-Neelsen staining of this mycobacterium revealed the presence of acid-fast bacilli that were finally identified as through 16S rDNA sequence analysis and a citrate utilization test. The current report may help other clinicians to make a quick and accurate diagnosis of RGM infection.
在过去十年中，已报告了越来越多的非结核分枝杆菌感染病例，尤其是由快速生长分枝杆菌（RGM）引起的病例。传统的分枝杆菌诊断方法效率低下，容易导致误诊。已报道了诸如基因测序等新的检测方法，但尚未广泛使用。本病例报告的目的是提供一种快速准确的感染鉴定方法。本研究报告的特定病例最初表现为高血糖和右腿丘疹。真菌常规培养多次呈阴性。然而，脓性物质在需氧培养五天后培养出一种快速生长的非结核分枝杆菌。对该分枝杆菌进行齐-尼氏染色显示存在抗酸杆菌，最终通过16S rDNA序列分析和柠檬酸盐利用试验鉴定为[具体菌种未给出]。本报告可能有助于其他临床医生对RGM感染做出快速准确的诊断。
