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分枝杆菌脓肿菌株的分子指纹图谱在一组小儿囊性纤维化患者中。

Molecular fingerprinting of Mycobacterium abscessus strains in a cohort of pediatric cystic fibrosis patients.

机构信息

Department of Microbiology, Virology and Infection Control, Great Ormond Street Hospital NHS Foundation Trust, London, United Kingdom.

出版信息

J Clin Microbiol. 2012 May;50(5):1758-61. doi: 10.1128/JCM.00155-12. Epub 2012 Mar 7.

Abstract

Forty-one Mycobacterium abscessus complex isolates from 17 pediatric cystic fibrosis (CF) patients were typed using a novel variable-number tandem repeat (VNTR) scheme and an automated repetitive-PCR (rep-PCR) system. Both VNTR and rep-PCR typing methods differentiate between members of the M. abscessus complex. The isolates from individual patients are indistinguishable, and the data strongly suggest that individual CF patients are persistently infected with one strain and also suggests that different CF patients can harbor the same strain.

摘要

从 17 例小儿囊性纤维化 (CF) 患者中分离出 41 株脓肿分枝杆菌复合群,采用新型可变数目串联重复 (VNTR) 方案和自动化重复序列 PCR (rep-PCR) 系统对其进行了分型。VNTR 和 rep-PCR 分型方法可区分脓肿分枝杆菌复合群的成员。来自单个患者的分离株无法区分,数据强烈表明,单个 CF 患者持续感染一种菌株,并表明不同 CF 患者可以携带相同的菌株。

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