Liu Ziru, Yang Zhulin, Liu Dongcai, Li Daiqiang, Zou Qiong, Yuan Yuan, Li Jinghe, Liang Lufeng, Chen Meigui, Chen Senlin
Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
Department of Pathology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
Oncol Lett. 2014 Apr;7(4):1128-1138. doi: 10.3892/ol.2014.1886. Epub 2014 Feb 14.
The clinicopathological characteristics of squamous cell/adenosquamous carcinoma (SC/ASC) are currently not well documented, and as the prevalence of SC/ASC is uncommon in gallbladder cancers, a prognostic marker has not yet been found. In the present study, the expression of tumor susceptibility gene (TSG) 101 and paternally expressed gene (PEG) 10 was assessed in 46 SC/ASCs and 80 adenocarcinomas (ACs) using immunohistochemistry, and the samples were further analyzed to examine correlations with the clinicopathological characteristics. It was demonstrated that positive TSG101 and PEG10 expression were significantly associated with large tumor size, high tumor-node-metastasis (TNM) stage, lymph node metastasis, invasion and no resection (only biopsy) of SC/ASC and AC. The univariate Kaplan-Meier analysis showed that positive TSG101 and PEG10 expression, and differentiation, tumor size, TNM stage, lymph node metastasis, invasion and surgical curability, is closely associated with a decreased overall survival in SC/ASC and AC patients (P<0.05 or P<0.001). The multivariate Cox regression analysis identified that positive TSG101 and PEG10 expression are independent factors for a poor-prognosis in SC/ASC and AC patients. The present study indicates that positive TSG101 and PEG10 expression are closely associated with the clinical, pathological and biological behaviors, and a poor prognosis in gallbladder cancer.
目前，鳞状细胞/腺鳞癌（SC/ASC）的临床病理特征尚未得到充分记录，而且由于SC/ASC在胆囊癌中的患病率较低，尚未发现其预后标志物。在本研究中，采用免疫组织化学方法评估了46例SC/ASC和80例腺癌（AC）中肿瘤易感基因（TSG）101和父系表达基因（PEG）10的表达情况，并对样本进行进一步分析以研究其与临床病理特征的相关性。结果表明，TSG101和PEG10的阳性表达与SC/ASC和AC的肿瘤体积大、肿瘤-淋巴结-转移（TNM）分期高、淋巴结转移、浸润以及未切除（仅活检）显著相关。单因素Kaplan-Meier分析显示，TSG101和PEG10的阳性表达、分化程度、肿瘤大小、TNM分期、淋巴结转移、浸润及手术可切除性与SC/ASC和AC患者的总生存期降低密切相关（P<0.05或P<0.001）。多因素Cox回归分析确定，TSG101和PEG10的阳性表达是SC/ASC和AC患者预后不良的独立因素。本研究表明，TSG101和PEG10的阳性表达与胆囊癌的临床、病理和生物学行为密切相关，且提示预后不良。
