Alam Md Maroof, Meerza Dilnasheen, Naseem Imrana
Department of Biochemistry, Faculty of Life Sciences, AMU, Aligarh 202002, India.
Department of Biochemistry, Faculty of Life Sciences, AMU, Aligarh 202002, India.
Life Sci. 2014 Jul 25;109(1):8-14. doi: 10.1016/j.lfs.2014.06.005. Epub 2014 Jun 16.
Quercetin is a natural polyphenolic flavonoid and acts as a quencher for reactive oxygen species generated by any physical or chemical action. In type 2 diabetes mellitus (T2DM) the basic characteristic feature is hyperglycemia which leads to complications involving oxidative stress. In view of this, the present study was conducted to examine the effect of quercetin in T2DM.
A total of 18 mice were divided into three groups, vis control, diabetic and diabetic treated with quercetin. Fasting blood glucose (FBG) levels and anti-oxidant enzyme activity were assayed. Creatinine, urea, lipid peroxidation, GLUT4 expression and DNA damage were also measured.
A significant decrease in FBG level and liver and kidney marker enzymes was observed in the quercetin treated group as compared to the diabetic one. Glutathione, SOD, catalase, and glutathione-S-transferase levels were also found to be increased on quercetin supplementation. Thiobarbituric acid-reactive substance level was decreased while GLUT4 expression levels were increased in the treated group. DNA damage was also affected positively by quercetin when subjected with single cell alkaline gel electrophoresis. Thus, we may suggest an anti-oxidant potential and protective effect of quercetin in T2DM mice.
From this study, we conclude that quercetin ameliorates hyperglycemia and oxidative stress, by blunting free radical induced toxicity in T2DM.
槲皮素是一种天然多酚类黄酮，可作为由任何物理或化学作用产生的活性氧的淬灭剂。在2型糖尿病（T2DM）中，基本特征是高血糖，这会导致涉及氧化应激的并发症。鉴于此，本研究旨在检测槲皮素对T2DM的影响。
总共18只小鼠被分为三组，即对照组、糖尿病组和槲皮素治疗的糖尿病组。检测空腹血糖（FBG）水平和抗氧化酶活性。还测量了肌酐、尿素、脂质过氧化、葡萄糖转运蛋白4（GLUT4）表达和DNA损伤。
与糖尿病组相比，槲皮素治疗组的FBG水平以及肝脏和肾脏标记酶显著降低。补充槲皮素后，谷胱甘肽、超氧化物歧化酶、过氧化氢酶和谷胱甘肽-S-转移酶水平也升高。治疗组中硫代巴比妥酸反应性物质水平降低，而GLUT4表达水平升高。当进行单细胞碱性凝胶电泳时，槲皮素对DNA损伤也有积极影响。因此，我们可以认为槲皮素对T2DM小鼠具有抗氧化潜力和保护作用。
从本研究中，我们得出结论，槲皮素通过减轻T2DM中自由基诱导的毒性来改善高血糖和氧化应激。
