Balasubramanian V, Natarajan K, Rajeshkannan V, Perumal P
Rhizosphere Biology Laboratory, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India,
Environ Sci Pollut Res Int. 2014 Nov;21(21):12549-62. doi: 10.1007/s11356-014-3191-2. Epub 2014 Jun 21.
Partially degraded high-density polyethylene (HDPE) was collected from plastic waste dump yard for biodegradation using fungi. Of various fungi screened, strain MF12 was found efficient in degrading HDPE by weight loss and Fourier transform infrared (FT-IR) spectrophotometric analysis. Strain MF12 was selected as efficient HDPE degraders for further studies, and their growth medium composition was optimized. Among those different media used, basal minimal medium (BMM) was suitable for the HDPE degradation by strain MF12. Strain MF12 was subjected to 28S rRNA sequence analysis and identified as Aspergillus terreus MF12. HDPE degradation was carried out using combinatorial physical and chemical treatments in conjunction to biological treatment. The high level of HDPE degradation was observed in ultraviolet (UV) and KMnO4/HCl with A. terreus MF12 treatment, i.e., FT10. The abiotic physical and chemical factors enhance the biodegradation of HDPE using A. terreus MF12.
从塑料垃圾场收集部分降解的高密度聚乙烯（HDPE），用于利用真菌进行生物降解。在筛选的各种真菌中，通过重量损失和傅里叶变换红外（FT-IR）分光光度分析发现菌株MF12在降解HDPE方面效率很高。选择菌株MF12作为高效HDPE降解菌进行进一步研究，并对其生长培养基成分进行了优化。在使用的那些不同培养基中，基础基本培养基（BMM）适合菌株MF12降解HDPE。对菌株MF12进行了28S rRNA序列分析，鉴定为土曲霉MF12。结合物理和化学处理与生物处理进行HDPE降解。在紫外线（UV）和高锰酸钾/盐酸与土曲霉MF12处理（即FT10）中观察到高水平的HDPE降解。非生物物理和化学因素增强了利用土曲霉MF12对HDPE的生物降解。
