AbouZid S
a Department of Pharmacognosy , Faculty of Pharmacy, University of Beni-Suef , Beni-Suef 62111 , Egypt.
Nat Prod Res. 2014;28(23):2102-10. doi: 10.1080/14786419.2014.927465. Epub 2014 Jun 20.
Plant cell culture can be a potential source for the production of important secondary metabolites. This technology bears many advantages over conventional agricultural methods. The main problem to arrive at a cost-effective process is the low productivity. This is mainly due to lack of differentiation in the cultured cells. Many approaches have been used to maximise the yield of secondary metabolites produced by cultured plant cells. Among these approaches: choosing a plant with a high biosynthetic capacity, obtaining efficient cell line for growth and production of metabolite of interest, manipulating culture conditions, elicitation, metabolic engineering and organ culture. This article gives an overview of the various approaches used to maximise the production of pharmaceutically important secondary metabolites in plant cell cultures. Examples of using these different approaches are shown for the production of silymarin from Silybum marianum tissue culture.
植物细胞培养可能是生产重要次生代谢产物的一个潜在来源。与传统农业方法相比，这项技术具有许多优势。实现具有成本效益的工艺的主要问题是生产率低下。这主要是由于培养细胞缺乏分化。人们已经采用了许多方法来最大化培养植物细胞产生的次生代谢产物的产量。在这些方法中：选择具有高生物合成能力的植物、获得用于生长和生产目标代谢物的高效细胞系、控制培养条件、诱导、代谢工程和器官培养。本文概述了用于最大化植物细胞培养中重要药用次生代谢产物产量的各种方法。展示了使用这些不同方法从水飞蓟组织培养中生产水飞蓟素的实例。
