Mallmann Adriano Olnei, Marchioro Alexandro, Oliveira Maurício Schneider, Rauber Ricardo Hummes, Dilkin Paulo, Mallmann Carlos Augusto
Laboratório de Análises Micotoxicológicas Departamento de Medicina Veterinária Preventiva Universidade Federal de Santa Maria Santa MariaRS Brazil.
Programa de Pós-graduação em Ciências Veterinárias Universidade Federal do Rio Grande do Sul Porto AlegreRS Brazil.
Braz J Microbiol. 2014 May 19;45(1):35-42. doi: 10.1590/s1517-83822014000100006. eCollection 2014.
Variance and performance of two sampling plans for aflatoxins quantification in maize were evaluated. Eight lots of maize were sampled using two plans: manual, using sampling spear for kernels; and automatic, using a continuous flow to collect milled maize. Total variance and sampling, preparation, and analysis variance were determined and compared between plans through multifactor analysis of variance. Four theoretical distribution models were used to compare aflatoxins quantification distributions in eight maize lots. The acceptance and rejection probabilities for a lot under certain aflatoxin concentration were determined using variance and the information on the selected distribution model to build the operational characteristic curves (OC). Sampling and total variance were lower at the automatic plan. The OC curve from the automatic plan reduced both consumer and producer risks in comparison to the manual plan. The automatic plan is more efficient than the manual one because it expresses more accurately the real aflatoxin contamination in maize.
评估了两种用于玉米中黄曲霉毒素定量的抽样方案的方差和性能。使用两种方案对八批玉米进行抽样：手动抽样，使用取样矛采集玉米粒；自动抽样，使用连续流收集磨碎的玉米。通过多因素方差分析确定并比较了两种方案之间的总方差以及抽样、制备和分析方差。使用四种理论分布模型比较了八批玉米中黄曲霉毒素定量分布。利用方差和所选分布模型的信息构建操作特征曲线（OC），确定了在特定黄曲霉毒素浓度下一批产品的接受和拒收概率。自动抽样方案的抽样方差和总方差较低。与手动方案相比，自动方案的OC曲线降低了消费者和生产者风险。自动方案比手动方案更有效，因为它能更准确地表达玉米中实际的黄曲霉毒素污染情况。
