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通过便捷引入AtpA-C252F突变快速提高蓝藻细胞工厂的高光和高温耐受性

Rapidly Improving High Light and High Temperature Tolerances of Cyanobacterial Cell Factories Through the Convenient Introduction of an AtpA-C252F Mutation.

作者信息

Zhang Shanshan, Zheng Sini, Sun Jiahui, Zeng Xuexia, Duan Yangkai, Luan Guodong, Lu Xuefeng

机构信息

Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.

Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.

出版信息

Front Microbiol. 2021 Apr 8;12:647164. doi: 10.3389/fmicb.2021.647164. eCollection 2021.

DOI:10.3389/fmicb.2021.647164
PMID:33897662
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8060558/
Abstract

Photosynthetic biomanufacturing is a promising route for green production of biofuels and biochemicals utilizing carbon dioxide and solar energy. Cyanobacteria are important microbial platforms for constructing photosynthetic cell factories. Toward scaled outdoor cultivations in the future, high light and high temperature tolerances of cyanobacterial chassis strains and cell factories would be determinant properties to be optimized. We proposed a convenient strategy for rapidly improving high light and high temperature tolerances of an important cyanobacterial chassis PCC 7942 and the derived cell factories. Through introduction and isolation of an AtpA-C252F mutation, PCC 7942 mutants with improved high light and high temperature tolerances could be obtained in only 4 days with an antibiotics-free mode. Adopting this strategy, cellular robustness and sucrose synthesizing capacities of a PCC 7942 cell factory were successfully improved.

摘要

光合生物制造是利用二氧化碳和太阳能绿色生产生物燃料和生化制品的一条很有前景的途径。蓝细菌是构建光合细胞工厂的重要微生物平台。对于未来规模化的户外培养而言，蓝细菌底盘菌株和细胞工厂的高光耐受性和高温耐受性将是需要优化的决定性特性。我们提出了一种便捷策略，用于快速提高重要蓝细菌底盘菌株PCC 7942及其衍生细胞工厂的高光耐受性和高温耐受性。通过引入和分离AtpA-C252F突变，仅需4天且无需抗生素模式就能获得高光耐受性和高温耐受性得到改善的PCC 7942突变体。采用该策略，成功提高了PCC 7942细胞工厂的细胞稳健性和蔗糖合成能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b1/8060558/ebb8a2867619/fmicb-12-647164-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b1/8060558/ba11abe6ddfe/fmicb-12-647164-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b1/8060558/4a511394df7b/fmicb-12-647164-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b1/8060558/2e296ce33348/fmicb-12-647164-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b1/8060558/ebb8a2867619/fmicb-12-647164-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b1/8060558/ba11abe6ddfe/fmicb-12-647164-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b1/8060558/4a511394df7b/fmicb-12-647164-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b1/8060558/2e296ce33348/fmicb-12-647164-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b1/8060558/ebb8a2867619/fmicb-12-647164-g004.jpg

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Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.视紫红质：一种用于研究、开发和创新工程的极具通用性的蛋白质种类。
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Comparative genomics reveals the molecular determinants of rapid growth of the cyanobacterium UTEX 2973.比较基因组学揭示了蓝藻 UTEX 2973 快速生长的分子决定因素。
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