Aziz Faisal, Chen Xin, Yang Xuesong, Yan Qiu
Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian, China.
Department of Surgery, 2nd Affiliated Hospital of Dalian Medical University, Dalian, China.
Biomed Res Int. 2014;2014:142980. doi: 10.1155/2014/142980. Epub 2014 May 13.
Helicobacter pylori vacA and cagA genes have significant genetic heterogenicity, resulting in different clinical outcomes. Northeast part of China has reported high prevalence of H. pylori infections and gastric cancer. Hence, we investigated the H. pylori cagA and vacA genotypes with clinical outcomes in Northeast China. Gastric tissue samples (n = 169), chronic gastritis (GIs), gastric ulcer (GU), and gastric cancer (GC) were analysed for 16S rRNA ureA, cagA, and cagA genotypes by PCR. A total of 141 (84%) cases were found positive for H. pylori by 16S rRNA and ureA. GC showed high H. pylori infection (93%) compared with GIs (72%) and GU (84%). The vacAs1am1 was highly found in GC (40%) and GU (36%), vacAs1am2 in GIs (33%), vacAs1bm1 (14%) and vacAs1bm2 (8%) in GU cases, and s2m1 in normal cases (33%), while vacAs1cm1 showed low frequency in GIs (2%) and GU (3%) and GC showed negative result. The East-Asian cagA strain was highly observed in GC (43%), as compared to GIs (41%) and GU (20%). The East-Asian cagA/vacAs1am1 was significantly higher in GC (23%) than in GU (22%) and GIs (145) patients. The East-Asian type cagA with vacAs1a and vacAm1 is the most predominant genotype in H. pylori strains of Northeast China.
幽门螺杆菌vacA和cagA基因具有显著的遗传异质性，导致不同的临床结果。中国东北地区报道幽门螺杆菌感染和胃癌的患病率较高。因此，我们在中国东北地区研究了幽门螺杆菌cagA和vacA基因型与临床结果的关系。通过聚合酶链反应（PCR）对胃组织样本（n = 169）、慢性胃炎（GI）、胃溃疡（GU）和胃癌（GC）进行16S rRNA、ureA、cagA和cagA基因型分析。通过16S rRNA和ureA共发现141例（84%）幽门螺杆菌阳性病例。与GI（72%）和GU（84%）相比，GC显示出较高的幽门螺杆菌感染率（93%）。vacAs1am1在GC（40%）和GU（36%）中高度检出，vacAs1am2在GI（33%）中检出，vacAs1bm1（14%）和vacAs1bm2（8%）在GU病例中检出，s2m1在正常病例中（33%）检出，而vacAs1cm1在GI（2%）和GU（3%）中出现频率较低，GC显示为阴性结果。与GI（41%）和GU（20%）相比，东亚cagA菌株在GC中高度观察到（43%）。东亚cagA/vacAs1am1在GC（23%）中显著高于GU（22%）和GI（145）患者。东亚型cagA与vacAs1a和vacAm1是中国东北地区幽门螺杆菌菌株中最主要的基因型。
