Mohtasham Nooshin, Saghravanian Nasrollah, Zare Reza, Saghafi Shadi, Ghazi Narges, Mohajertehran Farnaz, Shahabinejad Mehdi
Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Oral and Maxillofacial Pathology, School of Dentistry, Mashhad University of Medical Sciences,Mashhad, Iran.
Dent Res J (Isfahan). 2022 Jan 28;19:8. doi: 10.4103/1735-3327.336693. eCollection 2022.
The associations between and human papillomavirus (HPV) with head and neck squamous cell carcinomas (HNSCCs) are approved before. However, the association between demographic, clinicopathological, and histologic characteristics of HNSCC patients and molecular detection of HPV and has not been enough investigated.
In this cross-sectional study, 62 patients with HNSCC from January 2016 to February 2020 were entered the study. For detection 16S ribosomal RNA and glmM genes and HPV detection, MY09 and MY11 genes were used. < 0.05 is considered as significant level.
There were 34 patients with advanced-stage cancer (54.8%). Grade I patients (61.3%) had the highest frequency. There were 20 (32.25%) and 7 (11.29%) patients with positive infection among tumor tissue and healthy tissue margins, respectively. Positive HPV infections were in 8 (12.90%) and 3 (4.83%) patients, respectively, in tumor tissue and healthy tissue margins ( = 0.01). There was a significant difference between histological grade and infection to HPV among HNSCC patients ( = 0.01), and most of the positive HPV cases had well-, moderate-, and poorly-differentiated tumors, respectively. Our study showed a significant increase in HPV infection in the advanced-stage group compared to the early-stage group ( = 0.05).
Our study findings concluded a significant relationship between HPV infection in HNSCC patients with age, stage, and grade. In summary, our findings based on polymerase chain reaction analysis concluded remarkably a potential role of HPV infection and to some extent infection into the contribution of HNSCC malignancies.
此前已证实 与人类乳头瘤病毒（HPV）与头颈部鳞状细胞癌（HNSCC）之间存在关联。然而，HNSCC患者的人口统计学、临床病理和组织学特征与HPV及 的分子检测之间的关联尚未得到充分研究。
在这项横断面研究中，纳入了2016年1月至2020年2月期间的62例HNSCC患者。使用16S核糖体RNA和glmM基因进行 检测，使用MY09和MY11基因进行HPV检测。P < 0.05被视为显著水平。
有34例晚期癌症患者（54.8%）。I级患者的比例最高（61.3%）。肿瘤组织和健康组织边缘分别有20例（32.25%）和7例（11.29%）患者 感染呈阳性。肿瘤组织和健康组织边缘的HPV阳性感染分别为8例（12.90%）和3例（4.83%）患者（P = 0.01）。HNSCC患者的组织学分级与HPV感染之间存在显著差异（P = 0.01），大多数HPV阳性病例分别具有高分化、中分化和低分化肿瘤。我们的研究表明，与早期组相比，晚期组的HPV感染显著增加（P = 0.05）。
我们的研究结果得出，HNSCC患者的HPV感染与年龄、分期和分级之间存在显著关系。总之，我们基于聚合酶链反应分析的结果显著得出HPV感染以及在一定程度上 感染在HNSCC恶性肿瘤形成中的潜在作用。
