García-Nieto Edelmira, Juárez-Santacruz Libertad, García-Gallegos Elizabeth, Tlalmis-Zempoalteca Joselin, Romo-Gómez Claudia, Torres-Dosal Arturo
Centro de Investigación en Genética y Ambiente, Universidad Autónoma de Tlaxcala, Km 10.5 Autopista San Martín-Tlaxcala, CP 90120, Ixtacuixtla, Tlax., Mexico,
Bull Environ Contam Toxicol. 2014 Oct;93(4):393-8. doi: 10.1007/s00128-014-1318-2. Epub 2014 Jun 21.
This study evaluated the genotoxic impact of anthropic activities in Huactzinco Spring, using Cyprinus carpio as a biomonitor. In situ and in vivo experimental designs were compared by means of simultaneous 2-week exposures. The water from the spring generated mean micronuclei frequency values (108.6 ± 32 MN/1,000) and DNA fragmentation values (143.4 ± 35 au) which were statistically higher than those for the negative control (10.9 ± 6 MN/1,000 and 67.6 ± 23 au). The in situ and in vivo experiments supported one another. The comet assay proved to be the most sensitive test, with an EC50 value (11.4 % ± 3.4 %) being less than that determined for the micronuclei test (54.8 % ± 3.2 %). The results of this study confirm the usefulness of C. carpio as an environmental contamination biomonitor, and suggest that Huactzinco Spring water constitutes a latent risk to human health and the environment.
本研究以鲤鱼作为生物监测器，评估了瓦茨金科泉中人类活动产生的遗传毒性影响。通过为期2周的同步暴露，对原位和体内实验设计进行了比较。泉水产生的平均微核频率值（108.6±32个微核/1000个细胞）和DNA片段化值（143.4±35个任意单位）在统计学上高于阴性对照（10.9±6个微核/1000个细胞和67.6±23个任意单位）。原位和体内实验相互印证。彗星试验被证明是最敏感的测试，其半数效应浓度（EC50）值（11.4%±3.4%）低于微核试验所确定的值（54.8%±3.2%）。本研究结果证实了鲤鱼作为环境污染生物监测器的有效性，并表明瓦茨金科泉水对人类健康和环境构成潜在风险。
