Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Irbid, Jordan.
Drug Chem Toxicol. 2013 Oct;36(4):435-42. doi: 10.3109/01480545.2013.776577. Epub 2013 Mar 26.
The utilization of Schiff bases in the industrial and pharmaceutical fields has led to an increase in their syntheses and evaluation of their biological activities. In this study, we described the synthesis and genotoxicity of two Schiff bases that share common platform in their construction, namely, naphthalene, and are complexed to either Cu(II) or Zn(II). The genotoxicity of these complexes was evaluated in cultured lymphocytes using sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs), and in rats using the urine 8-OH-2-deoxyguanosine (8-OH-dG) assay. The results showed that the examined complexes are genotoxic, but with different degrees. The order of genotoxicity of the complexes at 10 µg/mL was: Cu(L3)(NCS)(H2O) > Zn(L3)(NCS)(H2O) > Cu(L2)(NCS) > Zn(L2)(NCS), where L2 and L3 are the conjugate bases of N-(8-quinolyl)napthaldimine and N-(anilinyl)napthaldimine, respectively. However, at the 1-µg/mL concentration, only the Cu(L3)(NCS)(H2O) complex induced significant CAs, whereas at the 0.1-µg/mL concentration, only Cu(L3)(NCS)(H2O) and Zn(L2)(NCS) complexes induced significant SCEs, compared to controls. In the urine 8-OH-dG assay, all complexes at 10 mg/100 g body weight (b.w.) were found to cause DNA damage with the following order: Cu(L3)(NCS)(H2O) > Zn(L2)(NCS) > Zn(L3)(NCS)(H2O) > Cu(L2)(NCS), whereas no significant DNA damage was observed in animals exposed to 1 and 0.1 mg/100 g b.w. (p > 0.05). In conclusion, the two examined Schiff base complexes are found to induce DNA damage, but with different degrees.
席夫碱在工业和制药领域的应用促进了它们的合成和生物活性评价。本研究描述了两种席夫碱的合成及其遗传毒性，它们在结构上有共同的平台，即萘，并与 Cu(II)或 Zn(II)配位。在培养的淋巴细胞中，用姐妹染色单体交换（SCE）和染色体畸变（CA）评估这些配合物的遗传毒性，在大鼠中用尿液 8-OH-2-脱氧鸟苷（8-OH-dG）测定法评估。结果表明，所研究的配合物具有遗传毒性，但程度不同。在 10μg/mL 时，配合物的遗传毒性顺序为：Cu(L3)(NCS)(H2O)＞Zn(L3)(NCS)(H2O)＞Cu(L2)(NCS)＞Zn(L2)(NCS)，其中 L2 和 L3 分别是 8-喹啉基萘二亚胺和 N-(苯胺基)萘二亚胺的共轭碱。然而，在 1μg/mL 浓度下，只有 Cu(L3)(NCS)(H2O)配合物诱导明显的 CA，而在 0.1μg/mL 浓度下，只有 Cu(L3)(NCS)(H2O)和 Zn(L2)(NCS)配合物诱导明显的 SCE，与对照组相比。在尿液 8-OH-dG 测定中，所有配合物在 10mg/100g 体重（bw）时均引起 DNA 损伤，其顺序为：Cu(L3)(NCS)(H2O)＞Zn(L2)(NCS)＞Zn(L3)(NCS)(H2O)＞Cu(L2)(NCS)，而在暴露于 1 和 0.1mg/100g bw 的动物中未观察到明显的 DNA 损伤（p＞0.05）。总之，两种被研究的席夫碱配合物均能引起 DNA 损伤，但程度不同。
