Alqudah Mohammad A Y, Al-Ashwal Fahmi Y, Alzoubi Karem H, Alkhatatbeh Mohammad, Khabour Omar
a Department of Clinical Pharmacy , Jordan University of Science and Technology , Irbid , Jordan.
b Department of Medical Laboratory Sciences , Jordan University of Science and Technology , Irbid , Jordan.
Drug Chem Toxicol. 2018 Jul;41(3):281-286. doi: 10.1080/01480545.2017.1384835. Epub 2017 Nov 2.
Oxaliplatin is a platinum-based anticancer drug that has been shown to be genotoxic to the normal cells. Vitamin E is a potent antioxidant that may protect and enhance the repair of the damaged DNA. In this study, we aimed to evaluate the genotoxic effect of oxaliplatin on DNA by measuring the frequency of chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) in cultured human lymphocytes. We also attempted to explore the potential protective effect of vitamin E on chromosomal damage induced by oxaliplatin. Results showed that oxaliplatin significantly increased the frequency of CAs (p < 0.001) and SCEs (p < 0.001) as compared to control. This chromosomal damage, caused by oxaliplatin, was significantly decreased by pretreatment of cells with vitamin E. Moreover, the results showed that oxaliplatin caused a significant reduction in the cell kinetic parameters, the mitotic index (MI) and the proliferative index (PI). However, vitamin E did not affect this reduction in the MI and PI. Therefore, we conclude that oxaliplatin is genotoxic, and vitamin E can prevent the chromosomal damage induced by oxaliplatin but it has no effect on oxaliplatin-induced cytotoxicity.
奥沙利铂是一种铂类抗癌药物，已被证明对正常细胞具有遗传毒性。维生素E是一种强效抗氧化剂，可能保护并增强受损DNA的修复。在本研究中，我们旨在通过测量培养的人淋巴细胞中的染色体畸变（CAs）频率和姐妹染色单体交换（SCEs）来评估奥沙利铂对DNA的遗传毒性作用。我们还试图探究维生素E对奥沙利铂诱导的染色体损伤的潜在保护作用。结果显示，与对照组相比，奥沙利铂显著增加了CAs频率（p < 0.001）和SCEs频率（p < 0.001）。用维生素E预处理细胞可显著降低奥沙利铂引起的这种染色体损伤。此外，结果表明奥沙利铂导致细胞动力学参数、有丝分裂指数（MI）和增殖指数（PI）显著降低。然而，维生素E并未影响MI和PI的这种降低。因此，我们得出结论，奥沙利铂具有遗传毒性，维生素E可预防奥沙利铂诱导的染色体损伤，但对奥沙利铂诱导的细胞毒性没有影响。
