维生素 B12 可预防顺铂诱导的遗传毒性。
Vitamin B12 Protects against Genotoxicity Induced by Cisplatin.
机构信息
Department of Applied Biological Sciences, Faculty of Science and Art, Jordan University of Science and Technology, Irbid, Jordan.
Department of Pharmacy Practice and Pharmacotherapeutics, College of Pharmacy, University of Sharjah, Sharjah, UAE.
出版信息
Curr Cancer Drug Targets. 2024;24(11):1169-1176. doi: 10.2174/0115680096284684240110044954.
BACKGROUND
Cisplatin is an effective synthetic chemotherapeutic drug used for cancer treatment. Vitamin B12 has been shown to possess anti-genotoxic activity. This study aimed to investigate the effect of vitamin B12 on chromosomal damage induced by cisplatin.
METHODS
The level of sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) were measured in cultured human blood lymphocytes treated with cisplatin and/or vitamin B12.
RESULTS
The results showed a significantly elevated frequency of CAs and SCEs of cisplatin-treated cultures compared to the control (P < 0.05). The CAs and SCEs induced by cisplatin were significantly lowered by pretreatment of cell cultures with vitamin B12. In addition, cisplatin caused a slight reduction in the mitotic index (MI), while vitamin B12 did not modulate the effect of cisplatin on MI.
CONCLUSION
Vitamin B12 can protect human lymphocytes against genotoxicity associated with cisplatin.
背景
顺铂是一种有效的合成化疗药物，用于癌症治疗。维生素 B12 已被证明具有抗基因毒性活性。本研究旨在探讨维生素 B12 对顺铂诱导的染色体损伤的影响。
方法
在培养的人血淋巴细胞中，用顺铂和/或维生素 B12 处理，测量姐妹染色单体交换（SCE）和染色体畸变（CA）的水平。
结果
结果显示，与对照组相比，顺铂处理的培养物中 CA 和 SCE 的频率显著升高（P < 0.05）。用维生素 B12 预处理细胞培养物可显著降低顺铂诱导的 CA 和 SCE。此外，顺铂轻微降低有丝分裂指数（MI），而维生素 B12 不调节顺铂对 MI 的影响。
结论
维生素 B12 可保护人淋巴细胞免受与顺铂相关的遗传毒性。
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