Xu Zhouying, Ban Yihui, Li Zhen, Chen Hui, Yang Ren, Tang Ming
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Environ Sci Pollut Res Int. 2014 Nov;21(22):12671-83. doi: 10.1007/s11356-014-3209-9. Epub 2014 Jun 25.
Understanding the influence of arbuscular mycorrhizal (AM) fungi on the expressions of the dominant plant-related genes under heavy metal (HM) stress is important for developing strategies to reclaim polluted sites. In this study, we cloned full-length cDNAs of phytochelatin synthase gene (PCS1) and Actin of Sophora viciifolia Hance., a predominant plant in Qiandongshan lead and zinc mine, by rapid amplification of cDNA ends. Consequently, we studied the response of SvPCS1 to Funneliformis mosseae inoculation under lead stress (0, 50, and 200 μM Pb(NO3)2) at different durations (1, 3, and 7 days) using quantitative reverse-transcription polymerase chain-reaction (qRT-PCR) technique. The Pb concentrations and chlorophyll fluorescence parameters were also measured to assay Pb toxicity to Sophora viciifolia. We found that Pb concentrations in roots increased with increasing Pb application and the durations; the F v /F m , F v /F o , qP, and Y(II) decreased; NPQ rose with increasing Pb concentrations; mycorrhizal symbiosis alleviated the Pb toxicity to plants; and SvPCS1 was constitutively expressed in the roots. It was also found that F. mosseae inoculation could promote the expression of SvPCS1 with the concentration ≤ 200 μM at the exposure time shorter than 7 days.
了解丛枝菌根（AM）真菌对重金属（HM）胁迫下主要植物相关基因表达的影响，对于制定污染场地修复策略具有重要意义。在本研究中，我们通过cDNA末端快速扩增技术，克隆了黔东山铅锌矿优势植物苦豆子的植物螯合素合酶基因（PCS1）和肌动蛋白的全长cDNA。随后，我们采用定量逆转录聚合酶链反应（qRT-PCR）技术，研究了在不同胁迫时间（1、3和7天）下，铅胁迫（0、50和200 μM Pb(NO3)2）条件下接种摩西管柄囊霉对苦豆子SvPCS1表达的影响。同时测定了铅浓度和叶绿素荧光参数，以分析铅对苦豆子的毒性。我们发现，根系中的铅浓度随着铅施用量和胁迫时间的增加而升高；F v /F m、F v /F o、qP和Y(II)降低；NPQ随铅浓度的增加而升高；菌根共生减轻了铅对植物的毒性；SvPCS1在根中组成型表达。还发现，在暴露时间短于7天、铅浓度≤200 μM时，接种摩西管柄囊霉可促进SvPCS1的表达。
