Vallelunga Annamaria, Ragusa Marco, Di Mauro Stefania, Iannitti Tommaso, Pilleri Manuela, Biundo Roberta, Weis Luca, Di Pietro Cinzia, De Iuliis Angela, Nicoletti Alessandra, Zappia Mario, Purrello Michele, Antonini Angelo
Molecular Neurobiology Laboratory, Department for Parkinson's Disease, IRCCS Hospital San Camillo Venice, Italy.
Unit of Molecular, Genome and Complex Systems BioMedicine, Department Gian Filippo Ingrassia, University of Catania Catania, Italy.
Front Cell Neurosci. 2014 Jun 10;8:156. doi: 10.3389/fncel.2014.00156. eCollection 2014.
Parkinson's disease (PD) is a progressive neurodegenerative disorder which may be misdiagnosed with atypical conditions such as Multiple System Atrophy (MSA), due to overlapping clinical features. MicroRNAs (miRNAs) are small non-coding RNAs with a key role in post-transcriptional gene regulation. We hypothesized that identification of a distinct set of circulating miRNAs (cmiRNAs) could distinguish patients affected by PD from MSA and healthy individuals. Results. Using TaqMan Low Density Array technology, we analyzed 754 miRNAs and found 9 cmiRNAs differentially expressed in PD and MSA patients compared to healthy controls. We also validated a set of 4 differentially expressed cmiRNAs in PD and MSA patients vs. controls. More specifically, miR-339-5p was downregulated, whereas miR-223(*), miR-324-3p, and mir-24 were upregulated in both diseases. We found cmiRNAs specifically deregulated in PD (downregulation of miR-30c and miR-148b) and in MSA (upregulation of miR-148b). Finally, comparing MSA and PD, we identified 3 upregulated cmiRNAs in MSA serum (miR-24, miR-34b, miR-148b). Conclusions. Our results suggest that cmiRNA signatures discriminate PD from MSA patients and healthy controls and may be considered specific, non-invasive biomarkers for differential diagnosis.
帕金森病（PD）是一种进行性神经退行性疾病，由于临床特征重叠，可能会被误诊为诸如多系统萎缩（MSA）等非典型病症。微小RNA（miRNA）是一类小的非编码RNA，在转录后基因调控中起关键作用。我们假设，鉴定一组独特的循环miRNA（cmiRNA）可以区分PD患者与MSA患者及健康个体。结果。使用TaqMan低密度阵列技术，我们分析了754种miRNA，发现与健康对照相比，有9种cmiRNA在PD和MSA患者中差异表达。我们还验证了一组在PD和MSA患者与对照中差异表达的4种cmiRNA。更具体地说，miR-339-5p下调，而miR-223（*）、miR-324-3p和mir-24在两种疾病中均上调。我们发现cmiRNA在PD（miR-30c和miR-148b下调）和MSA（miR-148b上调）中特异性失调。最后，比较MSA和PD，我们在MSA血清中鉴定出3种上调的cmiRNA（miR-24、miR-34b、miR-148b）。结论。我们的结果表明，cmiRNA特征可区分PD患者与MSA患者及健康对照，并且可被视为用于鉴别诊断的特异性非侵入性生物标志物。
