Shadab Mohammad, Agrawal Dhirendra Kumar, Aslam Mohammad, Islam Najmul, Ahmad Zuber
Assistant Professor, Department of Physiology, Jazan University , Kingdom of Saudi Arabia .
Professor, Department of Physiology, A.M.U. , Aligarh, UP, India .
J Clin Diagn Res. 2014 Apr;8(4):BC11-2. doi: 10.7860/JCDR/2014/5925.4291. Epub 2014 Apr 15.
Sewage workers, because of their occupation, are exposed to different types of dusts, bio-aerosols, fumes and gases like methane, hydrogen sulfide, sulphur dioxide, etc, which contribute towards oxidative stress and detrimental effects on various body functions, especially lung functions.
This study was carried out on sewage workers (who had been working for more than five years). We wanted to study the role of oxidative stress in development of impaired lung functions among sewage workers.
This cross sectional study was done in a tertiary care hospital (J.N. Medical College) in Aligarh, U.P. Study was done from March 2008 to December 2009. The study group comprised of 62 sewage workers who had been working for more than five years (32 non-smokers and 30 smokers) and 60 control subjects (30 smokers and 30 non-smokers). The pulmonary functions of these workers were assessed by using a MIR (Medical International Lab) Spiro Lab II Spirometer, with subjects in sitting position. Valid written consents were obtained from all the subjects. Malondialdehyde (MDA) is produced as a result of the action of reactive oxygen species (ROS) on the lipids present in the membranes of the cells, especially, contracting muscle cells. Serum MDA levels were assessed as an indirect measure of oxidative stress in these sewage workers and they were compared with serum MDA levels of control subjects. Appropriate statistical tests were applied for analysis of the data which was generated.
There were statistically significant decreases in Peak Expiratory Flow Rate (PEFR), Forced Expiratory Volume in first second (FEV1) and FEV1/FVC percent ratio (<80%) and Forced Expiratory Flow at 25%-75% of volume as percentage of Vital Capacity (FEF 25%-75%). Also, we found statistically significant increased levels of serum MDA in these sewage workers as compared to those in control subjects (with a p-value of <0.05 with a confidence interval of 95%).
Our study found that the occupational exposure of the sewage workers to harmful dust, fumes, gases and bio-aerosols contributed to oxidative stress among them. This oxidative stress was one of the mechanisms which led to the development of obstructive impairment of lung functions in these sewage workers.
由于职业原因，污水工人会接触到不同类型的粉尘、生物气溶胶、烟雾以及诸如甲烷、硫化氢、二氧化硫等气体，这些会导致氧化应激，并对身体的各种功能，尤其是肺功能产生有害影响。
本研究针对污水工人（工作年限超过五年）开展。我们旨在研究氧化应激在污水工人肺功能受损发展过程中的作用。
本横断面研究在北方邦阿利加尔的一家三级护理医院（J.N.医学院）进行。研究时间为2008年3月至2009年12月。研究组由62名工作年限超过五年的污水工人组成（32名非吸烟者和30名吸烟者）以及60名对照对象（30名吸烟者和30名非吸烟者）。这些工人的肺功能通过使用MIR（医学国际实验室）Spiro Lab II肺量计进行评估，受试者取坐姿。所有受试者均获得有效书面同意书。丙二醛（MDA）是活性氧（ROS）作用于细胞尤其是收缩肌细胞膜中的脂质而产生的。血清MDA水平作为这些污水工人氧化应激的间接指标进行评估，并与对照对象的血清MDA水平进行比较。对所产生的数据应用适当的统计检验进行分析。
呼气峰值流速（PEFR）、第一秒用力呼气量（FEV1）以及FEV1/FVC百分比（<80%）和25% - 75%肺活量时的用力呼气流量（FEF 25% - 75%）在统计学上有显著下降。此外，我们发现这些污水工人的血清MDA水平与对照对象相比在统计学上有显著升高（p值<0.05，置信区间为95%）。
我们的研究发现，污水工人职业性接触有害粉尘、烟雾、气体和生物气溶胶会导致他们体内的氧化应激。这种氧化应激是导致这些污水工人出现阻塞性肺功能损害的机制之一。
