O'Rourke Robert W, Meyer Kevin A, Neeley Christopher K, Gaston Garen D, Sekhri Palak, Szumowski Marek, Zamarron Brian, Lumeng Carey N, Marks Daniel L
Department of Surgery, University of Michigan Health System, Ann Arbor, Michigan, USA; Department of Surgery, Ann Arbor Veteran's Administration Hospital, Ann Arbor, Michigan, USA.
Obesity (Silver Spring). 2014 Oct;22(10):2109-14. doi: 10.1002/oby.20823. Epub 2014 Jun 24.
Natural killer (NK) cells are understudied in the context of metabolic disease and obesity. The goal of this study was to define the effect of NK cell ablation on systemic inflammation and glucose homeostasis in murine obesity.
A transgenic murine model was used to study the effect of NK cell ablation on systemic inflammation and glucose homeostasis in the context of diet-induced obesity using flow cytometry, QRTPCR, and glucose tolerance and insulin sensitivity testing.
NK cell ablation achieved a three to fourfold decrease in NK cells but had no effect on T-cell levels in adipose tissues and spleen. NK cell ablation was associated with decreased total macrophage infiltration in intra-abdominal adipose tissue, but macrophage infiltration in subcutaneous adipose tissue and spleen was unaffected. NK cell ablation was associated with modest improvement in insulin sensitivity but had no effect on tissue transcript levels of inflammatory cytokines.
NK cells play a role in promoting intra-abdominal adipose tissue macrophage infiltration and systemic insulin resistance in obesity.
在代谢性疾病和肥胖的背景下，自然杀伤（NK）细胞的研究较少。本研究的目的是确定NK细胞消融对小鼠肥胖中全身炎症和葡萄糖稳态的影响。
使用转基因小鼠模型，通过流式细胞术、定量逆转录聚合酶链反应（QRTPCR）以及葡萄糖耐量和胰岛素敏感性测试，研究NK细胞消融在饮食诱导肥胖背景下对全身炎症和葡萄糖稳态的影响。
NK细胞消融使NK细胞数量减少了三到四倍，但对脂肪组织和脾脏中的T细胞水平没有影响。NK细胞消融与腹内脂肪组织中总巨噬细胞浸润减少有关，但皮下脂肪组织和脾脏中的巨噬细胞浸润未受影响。NK细胞消融与胰岛素敏感性的适度改善有关，但对炎症细胞因子的组织转录水平没有影响。
NK细胞在肥胖中促进腹内脂肪组织巨噬细胞浸润和全身胰岛素抵抗方面发挥作用。
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