[利用铜绿假单胞菌外毒素A制备诊断性红细胞制剂]

[Production of an diagnostic erythrocyte agent from Pseudomonas aeruginosa exotoxin A].

作者信息

Egizova S A, Deriabin P N, Karal'nik B V, Kanapina A Sh, Moroz A F

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1988 Dec(12):38-41.

Abstract

The sensitization of formolized sheep red blood cells with exotoxin A by means of chromium chloride or glutaraldehyde is more effective with respect to their sensitivity in the passive hemagglutination test than loading by means of amidol, tannin and rivanol. The use of chromium chloride decreases the consumption of exotoxin A 2, 8, 16 and 16 times in comparison with the use of amidol, tannin, rivanol or glutaraldehyde respectively. The high specificity of erythrocyte diagnosticum obtained from exotoxin A by means of chromium chloride is indicated in the study of hyperimmune sera to 22 different antigens of enteric bacteria and staphylococci in the passive hemagglutination test and to 10 different enterobacterial and staphylococcal antigens in the antibody neutralization test.

摘要

通过氯化铬或戊二醛用外毒素A对甲醛化绵羊红细胞进行致敏，在被动血凝试验中的敏感性方面比用阿米酚、单宁和利凡诺进行负载更有效。与分别使用阿米酚、单宁、利凡诺或戊二醛相比，使用氯化铬可使外毒素A的消耗量分别降低2倍、8倍、16倍和16倍。在对22种不同肠道细菌和葡萄球菌抗原的超免疫血清进行被动血凝试验以及对10种不同肠道细菌和葡萄球菌抗原进行抗体中和试验的研究中，表明了通过氯化铬从外毒素A获得的红细胞诊断剂具有高特异性。