Fernández-Cruz E, Gelpi E, Longo N, González B, de la Morena M T, Montes M G, Roselló J, Ramis I, Suarez A, Fernández A
Department of Immunology, Hospital General Gregorio Marañón, Madrid, Spain.
AIDS. 1989 Feb;3(2):91-6. doi: 10.1097/00002030-198902000-00007.
We analysed the production of prostaglandin E2 (PGE2) by monocytes in 11 drug addicts with AIDS and opportunistic infections and in 13 controls. In three patients and six controls we isolated the PGE2 fraction from culture supernatants of purified monocytes (greater than 95%) incubated in medium containing (3H) arachidonic acid, using silicic acid columns. In two of three patients PGE2-fraction values (21.9 and 21.6 pmol/g protein) were significantly higher than controls (10 +/- 4). In eight AIDS patients and seven controls, PGE2 levels were determined by high-performance liquid chromatography and radioimmunoassay (HPLC and RIA). In three out of eight patients PGE2 levels were markedly higher (736, 419 and 208 pg/ml) than the mean + 2 s.d. values from controls (73 +/- 51). We tested the effect of PGE2 on the production of interleukin-2 by normal phytohemagglutinin-stimulated peripheral blood lymphocytes (PBL). There was a significant suppression of interleukin-2 production by PGE2 released from AIDS monocytes. PGE2 may be one of the factors involved in the severe immune dysfunction associated with AIDS.
我们分析了11例患有艾滋病及机会性感染的吸毒者和13名对照者单核细胞中前列腺素E2（PGE2）的产生情况。在3例患者和6名对照者中，我们使用硅酸柱从在含（3H）花生四烯酸的培养基中孵育的纯化单核细胞（>95%）的培养上清液中分离出PGE2组分。在3例患者中的2例中，PGE2组分值（21.9和21.6 pmol/g蛋白）显著高于对照者（10±4）。在8例艾滋病患者和7名对照者中，通过高效液相色谱法和放射免疫测定法（HPLC和RIA）测定PGE2水平。在8例患者中的3例中，PGE2水平明显高于对照者的均值+2标准差（73±51）（分别为736、419和208 pg/ml）。我们测试了PGE2对正常植物血凝素刺激的外周血淋巴细胞（PBL）产生白细胞介素-2的影响。艾滋病单核细胞释放的PGE2对白细胞介素-2的产生有显著抑制作用。PGE2可能是与艾滋病相关的严重免疫功能障碍所涉及的因素之一。
