Surlin Petra, Silosi Isabela, Rauten Anne Marie, Cojocaru Manole, Foia Lili
Department of Periodontology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
Department of Immunology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
ScientificWorldJournal. 2014;2014:421029. doi: 10.1155/2014/421029. Epub 2014 May 20.
In the present study the aim was to measure the levels of Thrombospondin-1 (TSP1) and Lipocalin-2/matrix metalloproteinase 9 (MMP9/NGAL) complex in gingival crevicular fluid (GCF) at different time points of orthodontic treatment, to determine the relationship between these values and those of total-matrix metalloproteinase 9 (MMP9) and theirs implication in angiogenesis balance, in the situation of a good control of the bacterial plaque, emphasizing the role of TSP1 and MMP9/NGAL complex. GCF samples were collected from 16 young orthodontic patients requiring upper canine distalization (test tooth) with first premolar extraction. The contralateral canine (control tooth) was free from orthodontic force. For the orthodontic appliance, brackets Roth 0.018 inch with 0.012 inch NiTi archwire and a laceback were used. TSP1, MMP9/NGAL, and MMP9 increased from 1 hour before activation of orthodontic appliance to a maximum at 8 hours for MMP9 and 72 hours for MMP9/NGAL and TSP1. The results show a change in time of TSP1, MMP9/NGAL, and MMP9 levels in GCF of patients with this method of orthodontic treatment. The powerful correlation of MMP9/NGAL with TSP1 suggests their stronger involvement in angiogenesis processes in PDL during orthodontic periodontal remodeling, in the situation of a healthy periodontium and a good control of the bacterial plaque.
在本研究中，目的是测量正畸治疗不同时间点龈沟液（GCF）中血小板反应蛋白-1（TSP1）和脂质运载蛋白-2/基质金属蛋白酶9（MMP9/NGAL）复合物的水平，确定这些值与总基质金属蛋白酶9（MMP9）的值之间的关系及其在血管生成平衡中的意义，在菌斑得到良好控制的情况下，强调TSP1和MMP9/NGAL复合物的作用。从16名需要拔除第一前磨牙进行上颌尖牙远中移动（试验牙）的年轻正畸患者中采集GCF样本。对侧尖牙（对照牙）未受正畸力作用。正畸矫治器采用Roth 0.018英寸托槽和0.012英寸镍钛弓丝以及回扎线。从正畸矫治器激活前1小时到MMP9在8小时达到最大值、MMP9/NGAL和TSP1在72小时达到最大值期间，TSP1、MMP9/NGAL和MMP9均升高。结果显示，采用这种正畸治疗方法的患者GCF中TSP1、MMP9/NGAL和MMP9水平随时间发生变化。MMP9/NGAL与TSP1的强相关性表明，在健康牙周组织和菌斑良好控制的情况下，它们在正畸牙周重塑过程中对牙周膜血管生成过程的参与更强。
