Uraki Satoko, Sugimoto Kazushi, Shiraki Katsuya, Tameda Masahiko, Inagaki Yuji, Ogura Suguru, Kasai Chika, Nojiri Keiichiro, Yoneda Misao, Yamamoto Norihiko, Takei Yoshiyuki, Nobori Tsutomu, Ito Masaaki
First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
Department of Gastroenterology, Mie University School of Medicine, Tsu, Japan.
Int J Oncol. 2014 Sep;45(3):1059-64. doi: 10.3892/ijo.2014.2507. Epub 2014 Jun 20.
The innate immune system plays an important role as the first line of defense against many types of microbes. Accumulating reports suggest that human β-defensins (hBDs) are expressed by and have certain roles in some cancer cells. In this study, we investigated the roles of hBD-3 in colon cancer cells. The expression of hBD-3 was examined by reverse transcriptase-polymerase chain reaction analysis of colon cancer cell lines and immunohistochemical staining of colon cancer tissues. The effect of hBD-3 on proliferation of colon cancer was assessed using the MTT assay and a real-time cell analyzer, and the effect of hBD-3 on the migration of colon cancer cells was also examined. The results showed that hBD-3 is not expressed in colon cancer cells but is produced by tumor-infiltrating monocytes. Migration of colon cancer cells was significantly inhibited by hBD-3 in a dose-dependent manner, although proliferation of colon cancer cells was not affected by administration of hBD-3. Moreover, reduced expression of metastasis-associated 1 family, member 2 (MTA2) mRNA in colon cancer cells was associated with exposure to hBD-3. In conclusion, progression of colon cancer was inhibited by hBD-3 in a paracrine fashion. Therefore, hBD-3 may be a potent new agent for treating colon cancer.
先天免疫系统作为抵御多种微生物的第一道防线发挥着重要作用。越来越多的报道表明，人类β-防御素（hBDs）由某些癌细胞表达并在其中发挥一定作用。在本研究中，我们调查了hBD-3在结肠癌细胞中的作用。通过对结肠癌细胞系进行逆转录聚合酶链反应分析以及对结肠癌组织进行免疫组织化学染色来检测hBD-3的表达。使用MTT法和实时细胞分析仪评估hBD-3对结肠癌细胞增殖的影响，并检测hBD-3对结肠癌细胞迁移的影响。结果显示，hBD-3在结肠癌细胞中不表达，而是由肿瘤浸润单核细胞产生。hBD-3以剂量依赖的方式显著抑制结肠癌细胞的迁移，尽管给予hBD-3对结肠癌细胞的增殖没有影响。此外，结肠癌细胞中转移相关蛋白1家族成员2（MTA2）mRNA表达的降低与暴露于hBD-3有关。总之，hBD-3以旁分泌方式抑制结肠癌的进展。因此，hBD-3可能是一种治疗结肠癌的有效新药。
