Anastassiou E D, Karakiulakis G, Missirlis E, Maragoudakis M E, Dimitracopoulos G
Department of Microbiology, University of Patras Medical School, Greece.
J Clin Microbiol. 1989 Mar;27(3):490-4. doi: 10.1128/jcm.27.3.490-494.1989.
Alginate from a heavily mucoid Pseudomonas aeruginosa strain and slime glycolipoprotein obtained from the revertant nonmucoid variant of the mucoid strain were tested for mitogenic activity on human peripheral lymphocytes and for degradation of 3H-labeled basement membranes of the anterior lens capsule of bovine eyes. Slime glycolipoprotein exerted mitogenic activity in concentrations from 50 to 200 micrograms/ml, whereas alginate was not mitogenic as shown by [3H]thymidine uptake. Alginate did not show any basement membrane degradation, whereas slime glycolipoprotein exhibited basement-membrane-degrading activity from 35 to 450 micrograms/ml in a dose-related manner. This activity was inhibited by metal chelators but not thiol protease inhibitors. The results suggest that alginate lacks the mitogenic and biodegrading activities of slime glycolipoprotein; these activities nevertheless need further investigation.
对来自一株高度黏液样铜绿假单胞菌的藻酸盐以及从该黏液样菌株的回复突变非黏液样变体获得的黏液糖脂蛋白，进行了对人外周血淋巴细胞的促有丝分裂活性测试以及对牛眼前囊膜的3H标记基底膜的降解测试。黏液糖脂蛋白在浓度为50至200微克/毫升时具有促有丝分裂活性，而藻酸盐如通过[3H]胸腺嘧啶核苷摄取所显示的那样没有促有丝分裂活性。藻酸盐未显示出任何基底膜降解，而黏液糖脂蛋白在35至450微克/毫升的浓度范围内以剂量相关方式表现出基底膜降解活性。这种活性被金属螯合剂抑制，但不被巯基蛋白酶抑制剂抑制。结果表明藻酸盐缺乏黏液糖脂蛋白的促有丝分裂和生物降解活性；不过这些活性仍需进一步研究。
