Bartell P F, Krikszens A
Infect Immun. 1980 Mar;27(3):777-83. doi: 10.1128/iai.27.3.777-783.1980.
Glycolipoprotein, a purified fraction of the exopolysaccharide slime of Pseudomonas aeruginosa, was identified as responsible for a number of the biological activities of viable cells, including toxicity and immunogenicity capable of stimulating protective antibody against the lethal effects of viable cells. Antiserum against glycolipoprotein also mediated the phagocytosis and subsequent killing of viable P. aeruginosa by unstimulated mouse peritoneal macrophages. In the absence of anti-glycolipoprotein serum, macrophages did not significantly reduce the number of bacteria. The presence of complement in the experimental mixture did not affect the reduction of bacteria by the macrophage in the presence of anti-glycolipoprotein serum. The limiting effect of antiserum concentration on macrophage activity was studied, and maximal activity was found at 2%, with no further increase in activity at 5% Preopsonization of the bacteria with anti-glycolipoprotein serum had little effect on the course of phagocytosis within the experimental conditions. Variations in bacterium-to-macrophage input ratios, ranging from 30:1 to 1:30, did not affect the capacity of the macrophages for phagocytosis.
糖脂蛋白是铜绿假单胞菌胞外多糖黏液的一种纯化组分，被确定为具有许多活细胞的生物学活性，包括毒性和免疫原性，能够刺激产生针对活细胞致死效应的保护性抗体。抗糖脂蛋白抗血清还介导未受刺激的小鼠腹腔巨噬细胞对活的铜绿假单胞菌的吞噬作用及随后的杀伤作用。在没有抗糖脂蛋白血清的情况下，巨噬细胞不会显著减少细菌数量。实验混合物中补体的存在并不影响在抗糖脂蛋白血清存在时巨噬细胞对细菌的减少作用。研究了抗血清浓度对巨噬细胞活性的限制作用，发现最大活性出现在2%，5%时活性不再进一步增加。在实验条件下，用抗糖脂蛋白血清对细菌进行预调理对吞噬过程影响很小。细菌与巨噬细胞输入比例在30:1至1:30范围内变化时，并不影响巨噬细胞的吞噬能力。
