Fukushima Makoto, Saunders Richard C, Mullarkey Matthew, Doyle Alexandra M, Mishkin Mortimer, Fujii Naotaka
Laboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892, USA.
Laboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892, USA.
J Neurosci Methods. 2014 Aug 15;233:155-65. doi: 10.1016/j.jneumeth.2014.06.022. Epub 2014 Jun 24.
Electrocorticography (ECoG) permits recording electrical field potentials with high spatiotemporal resolution over a large part of the cerebral cortex. Application of chronically implanted ECoG arrays in animal models provides an opportunity to investigate global spatiotemporal neural patterns and functional connectivity systematically under various experimental conditions. Although ECoG is conventionally used to cover the gyral cortical surface, recent studies have shown the feasibility of intrasulcal ECoG recordings in macaque monkeys.
Here we developed a new ECoG array to record neural activity simultaneously from much of the medial and lateral cortical surface of a single hemisphere, together with the supratemporal plane (STP) of the lateral sulcus in macaque monkeys. The ECoG array consisted of 256 electrodes for bipolar recording at 128 sites.
We successfully implanted the ECoG array in the left hemisphere of three rhesus monkeys. The electrodes in the auditory and visual cortex detected robust event related potentials to auditory and visual stimuli, respectively. Bipolar recording from adjacent electrode pairs effectively eliminated chewing artifacts evident in monopolar recording, demonstrating the advantage of using the ECoG array under conditions that generate significant movement artifacts.
Compared with bipolar ECoG arrays previously developed for macaque monkeys, this array significantly expands the number of cortical target areas in gyral and intralsulcal cortex.
This new ECoG array provides an opportunity to investigate global network interactions among gyral and intrasulcal cortical areas.
皮质脑电图（ECoG）能够在大脑皮层的大部分区域以高时空分辨率记录电场电位。在动物模型中应用长期植入的ECoG阵列，为在各种实验条件下系统地研究整体时空神经模式和功能连接提供了机会。尽管ECoG传统上用于覆盖脑回皮质表面，但最近的研究表明在猕猴中进行脑沟内ECoG记录是可行的。
在此，我们开发了一种新的ECoG阵列，用于同时记录猕猴单个半球大部分内侧和外侧皮质表面以及外侧沟颞上平面（STP）的神经活动。该ECoG阵列由256个电极组成，用于在128个位点进行双极记录。
我们成功地将ECoG阵列植入三只恒河猴的左半球。听觉和视觉皮层中的电极分别检测到对听觉和视觉刺激的强烈事件相关电位。来自相邻电极对的双极记录有效消除了单极记录中明显的咀嚼伪迹，证明了在产生明显运动伪迹的条件下使用ECoG阵列的优势。
与先前为猕猴开发的双极ECoG阵列相比，该阵列显著扩大了脑回和脑沟内皮质中皮质目标区域的数量。
这种新的ECoG阵列提供了一个机会来研究脑回和脑沟内皮质区域之间的整体网络相互作用。
