Ledochowitsch P, Yazdan-Shahmorad A, Bouchard K E, Diaz-Botia C, Hanson T L, He J-W, Seybold B A, Olivero E, Phillips E A K, Blanche T J, Schreiner C E, Hasenstaub A, Chang E F, Sabes P N, Maharbiz M M
The UC Berkeley-UCSF Graduate Program in Bioengineering, Berkeley, CA, United States; The Center for Neural Engineering and Prostheses (CNEP), United States.
UCSF Center for Integrative Neuroscience, San Francisco, CA, United States; The Center for Neural Engineering and Prostheses (CNEP), United States.
J Neurosci Methods. 2015 Dec 30;256:220-31. doi: 10.1016/j.jneumeth.2015.07.028. Epub 2015 Aug 19.
To dissect the intricate workings of neural circuits, it is essential to gain precise control over subsets of neurons while retaining the ability to monitor larger-scale circuit dynamics. This requires the ability to both evoke and record neural activity simultaneously with high spatial and temporal resolution.
In this paper we present approaches that address this need by combining micro-electrocorticography (μECoG) with optogenetics in ways that avoid photovoltaic artifacts.
We demonstrate that variations of this approach are broadly applicable across three commonly studied mammalian species - mouse, rat, and macaque monkey - and that the recorded μECoG signal shows complex spectral and spatio-temporal patterns in response to optical stimulation.
While optogenetics provides the ability to excite or inhibit neural subpopulations in a targeted fashion, large-scale recording of resulting neural activity remains challenging. Recent advances in optical physiology, such as genetically encoded Ca(2+) indicators, are promising but currently do not allow simultaneous recordings from extended cortical areas due to limitations in optical imaging hardware.
We demonstrate techniques for the large-scale simultaneous interrogation of cortical circuits in three commonly used mammalian species.
为剖析神经回路的复杂运作机制，在保留监测更大规模回路动态能力的同时，精确控制神经元亚群至关重要。这需要具备以高空间和时间分辨率同时诱发和记录神经活动的能力。
在本文中，我们介绍了通过将微电极脑电图（μECoG）与光遗传学相结合来满足这一需求的方法，这些方法可避免光生伏打伪迹。
我们证明，这种方法的变体广泛适用于三种常用的哺乳动物——小鼠、大鼠和猕猴，并且记录到的μECoG信号在受到光刺激时呈现出复杂的频谱和时空模式。
虽然光遗传学能够以靶向方式激发或抑制神经亚群，但对由此产生的神经活动进行大规模记录仍然具有挑战性。光学生理学的最新进展，如基因编码的Ca(2+)指示剂，很有前景，但由于光学成像硬件的限制，目前还无法从扩展的皮质区域进行同步记录。
我们展示了在三种常用哺乳动物中对皮质回路进行大规模同步研究的技术。
