Kilic Sevtap, Yuksel Beril, Pinarli F, Albayrak A, Boztok B, Delibasi T
Kastamonu School of Medicine, Hacettepe University, Süleyman bey sokak 29/10, Maltepe, Ankara, 06420, Turkey,
J Assist Reprod Genet. 2014 Aug;31(8):975-82. doi: 10.1007/s10815-014-0268-2. Epub 2014 Jun 29.
We evaluate the effect of stem cells to induce endometrial proliferation and angiogenesis on Asherman Syndrome (AS).
The experimental study was performed in stemcell research laboratory. Forty Wistar-Albino rats were divided according to groups. In group1 (n = 10) to establish the model; trichloroacetic acid was injected to right uterine horn. Two weeks later, intrauterine synechia was confirmed. In group2 (n = 10), 2 weeks later, 2 × 106 mesenchymal stem cells (MSC) were injected into right uterine horn followed by three intraperitoneal injections of MSCs. In group3 (n = 10), daily oral estrogen was initiated on the second week. In group4 (n = 10), MSC injections and oral estrogen was given together. The amount of fibrosis, vascularisation, inflammation and immunohistochemical staining with vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA) and Ki-67 were evaluated in the uterine tissues.
In all treatment groups; fibrosis decreased but vascularisation and immunhistohemical stainings increased in the experimental side. The amount of fibrosis, vascularisation, Ki-67 and PCNA scores were similar between group2 and 3. In group4, comparing to group2, less fibrosis but more Ki-67, PCNA and VEGF staining was observed.
Stem cells, when added to estrogen, are a highly effective alternative to induce regeneration of endometrium in Asherman Syndrome therapy.
我们评估干细胞对阿谢曼综合征（AS）子宫内膜增殖和血管生成的诱导作用。
实验研究在干细胞研究实验室进行。40只Wistar - 白化大鼠按组划分。在第1组（n = 10）中建立模型；向右侧子宫角注射三氯乙酸。两周后，确认宫腔粘连。在第2组（n = 10）中，两周后向右侧子宫角注射2×10⁶间充质干细胞（MSC），随后腹腔注射三次MSC。在第3组（n = 10）中，从第二周开始每日口服雌激素。在第4组（n = 10）中，同时给予MSC注射和口服雌激素。评估子宫组织中的纤维化、血管化、炎症程度以及血管内皮生长因子（VEGF）、增殖细胞核抗原（PCNA）和Ki - 67的免疫组化染色情况。
在所有治疗组中；实验侧的纤维化减少，但血管化和免疫组化染色增加。第2组和第3组之间的纤维化、血管化、Ki - 67和PCNA评分相似。在第4组中，与第2组相比，观察到纤维化较少，但Ki - 67、PCNA和VEGF染色较多。
在阿谢曼综合征治疗中，干细胞与雌激素联合使用是诱导子宫内膜再生的一种高效替代方法。
