Jiang Shou-Fu, Zhang Xiao-Ping, Li Bao-Liang, He Yan-Yan, Liu Jing, Tang Yue-Hong, Cai Li
Southeast Asian J Trop Med Public Health. 2014 May;45(3):568-75.
Shistosomiasis is one of the important parasitic diseases in developing countries and especially remains a threat to public health in China. Many immunodiagnostic kits have shown cross-reactions with other parasitic diseases and need large volumes of serum for the tests. In this study, we evaluated partially purified soluble egg antigen (SEA) in a colloidal gold immunochromatography assay (GICA) card kit for rapid detection of anti-Schistosoma japonicum antibodies using 5 microl of serum. Partially purified SEA from S. japonica was purified by Sephacryl S-300 chromatography. The optional reaction system and detection level of GICA using partially purified SEA were established by improving conjugated concentration and formulation of sample buffer and labeled solution. GICA showed 93.7% sensitivity in detecting schistosomiasis patients, 97.6% specificity in healthy population and patients with other parasitic diseases and a Youden's index value of 0.91. Cross-reaction with other parasitic diseases, such as paragonimiasis (1 case) and toxoplasmosis (1 case) is significantly lower compared to using crude SEA. Partially purified SEA in GICA is practical for detection of schistosomiasis in the field as it requires a small volume of serum, has high sensitivity, and has low cross-reaction rate.
血吸虫病是发展中国家重要的寄生虫病之一，在中国尤其仍然对公共卫生构成威胁。许多免疫诊断试剂盒已显示出与其他寄生虫病的交叉反应，并且检测需要大量血清。在本研究中，我们评估了一种胶体金免疫层析分析（GICA）卡试剂盒中的部分纯化可溶性虫卵抗原（SEA），用于使用5微升血清快速检测抗日本血吸虫抗体。来自日本血吸虫的部分纯化SEA通过Sephacryl S - 300层析进行纯化。通过改进结合物浓度以及样品缓冲液和标记溶液的配方，建立了使用部分纯化SEA的GICA的最佳反应体系和检测水平。GICA在检测血吸虫病患者时灵敏度为93.7%，在健康人群和其他寄生虫病患者中的特异性为97.6%，约登指数值为0.91。与使用粗SEA相比，与其他寄生虫病如肺吸虫病（1例）和弓形虫病（1例）的交叉反应显著降低。GICA中的部分纯化SEA在现场检测血吸虫病方面具有实用性，因为它需要少量血清，具有高灵敏度且交叉反应率低。
