Veterinary Virology and Animal Viral Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
Research Unit of Epidemiology and Risk Analysis Applied to Veterinary Science (UREAR-ULg), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
Vet Microbiol. 2014 Aug 27;172(3-4):407-14. doi: 10.1016/j.vetmic.2014.06.004. Epub 2014 Jun 10.
Zoonotic transmission of hepatitis E virus (HEV) is of special concern, particularly in high income countries were waterborne infections are less frequent than in developing countries. High HEV seroprevalences can be found in European pig populations. The aims of this study were to obtain prevalence data on HEV infection in swine in Belgium and to phylogenetically compare Belgian human HEV sequences with those obtained from swine. An ELISA screening prevalence of 73% (95% CI 68.8-77.5) was determined in Belgian pigs and a part of the results were re-evaluated by Western blot (WB). A receiver operating characteristic curve analysis was performed and scenarios varying the ELISA specificity relative to WB were analysed. The seroprevalences estimated by the different scenarios ranged between 69 and 81% and are in agreement with the high exposure of the European pig population to HEV. Pig HEV sequences were genetically compared to those detected in humans in Belgium and a predominance of genotype 3 subtype f was shown in both swine and humans. The high HEV seroprevalence in swine and the close phylogenetic relationships between pig and human HEV sequences further support the risk for zoonotic transmission of HEV between humans and pigs.
人畜共患的戊型肝炎病毒 (HEV) 传播尤其值得关注，特别是在高收入国家，那里的水源性感染比发展中国家更为少见。欧洲猪群中存在高的 HEV 血清流行率。本研究的目的是在比利时获得猪群中 HEV 感染的流行率数据，并对从猪中获得的比利时人类 HEV 序列进行系统发育比较。在比利时猪中，通过酶联免疫吸附试验 (ELISA) 筛查出 73%（95%CI 68.8-77.5）的流行率，部分结果通过 Western blot（WB）进行了重新评估。进行了受试者工作特征曲线分析，并分析了 ELISA 特异性相对于 WB 的变化情况。不同方案估计的血清流行率在 69%至 81%之间，与欧洲猪群对 HEV 的高暴露情况一致。猪 HEV 序列与在比利时人类中检测到的序列进行了遗传比较，结果表明基因型 3 亚型 f 在猪和人类中均占优势。猪群中 HEV 的高血清流行率以及猪和人类 HEV 序列之间的密切系统发育关系进一步支持了 HEV 在人类和猪之间发生人畜共患传播的风险。
