Vasilyev F F, Silkov A N, Sennikov S V
Cell Mol Immunol. 2015 Mar;12(2):222-30. doi: 10.1038/cmi.2014.43. Epub 2014 Jun 30.
The biological activity of the multifunctional cytokine interleukin-1 (IL-1) is mediated by its receptors. The aim of this study was to determine if an association exists between single nucleotide polymorphisms (SNPs) in the IL-1 type 1 and 2 receptor genes (IL1R1 and IL1R2) and the expression level of membrane-bound IL1Rs on subpopulations of mononuclear cells or serum levels of soluble IL-1 receptors. It was observed that healthy individuals with the genotype TT in SNP rs2234650:C>T had a lower percentage of intact CD14(+) monocytes expressing IL1R1 on their surface. The SNP rs4141134:T>C in IL1R2 has also been associated with the percentage of intact CD3(+) T cells expressing IL1R2. Furthermore, individuals carrying the CC allele of SNP rs4141134:T>C and the TT allele of SNP rs2071008:T>G in IL1R2 had a lower density of IL1R2s on the surface of CD14(+) monocytes in lipopolysaccharide (LPS)-stimulated PBMC cultures. In summary, this study demonstrated that IL-1 receptor gene polymorphisms could be one of the factors influencing the expression of membrane-bound IL-1 receptors (IL1R) on immunocompetent cells.
多功能细胞因子白细胞介素-1（IL-1）的生物学活性由其受体介导。本研究的目的是确定白细胞介素-1 1型和2型受体基因（IL1R1和IL1R2）中的单核苷酸多态性（SNP）与单核细胞亚群上膜结合型IL1R的表达水平或可溶性IL-1受体的血清水平之间是否存在关联。观察到，在单核苷酸多态性rs2234650：C>T中基因型为TT的健康个体，其表面表达IL1R1的完整CD14(+)单核细胞百分比更低。IL1R2中的单核苷酸多态性rs4141134：T>C也与表达IL1R2的完整CD3(+)T细胞百分比有关。此外，在脂多糖（LPS）刺激的外周血单核细胞（PBMC）培养物中，携带IL1R2中rs4141134：T>C的CC等位基因和rs2071008：T>G的TT等位基因的个体，其CD14(+)单核细胞表面的IL1R2密度更低。总之，本研究表明，IL-1受体基因多态性可能是影响免疫活性细胞上膜结合型IL-1受体（IL1R）表达的因素之一。
