湿度和氧气水平对软X射线及低能电子诱导的DNA损伤的作用
The Role of Humidity and Oxygen Level on Damage to DNA Induced by Soft X-rays and Low-Energy Electrons.
作者信息
Alizadeh Elahe, Sanche Léon
机构信息
Group of Radiation Sciences, Department of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Québec, Canada J1H5N4.
出版信息
J Phys Chem C Nanomater Interfaces. 2013 Jul 9;117(43):22445-22453. doi: 10.1021/jp403350j.
Abstract
Single- and double-strand breaks induced by soft X-rays (1.5 keV) and photo-emitted LEEs (0-30 eV) were measured in dry and humid thin films of plasmid DNA irradiated under different controllable levels of oxygen at standard ambient temperature and pressure (SATP). values derived from these experiments shows that the presence of HO and changing the atmosphere from N to O, while keeping all other experimental parameters constant, increases the formation of DSBs by factors of 4.5 and 11.8 for X-rays and LEEs, respectively. Under an oxygenated environment in humid DNA films, the additional LEE-induced damage resulting from the combination of water and oxygen exhibits a supper-additive effect, which leads to the formation of DSBs with a value almost 7 times higher than that obtained by X-ray photons. These results indicate that O, HO and LEEs effectively contribute synergistically to enhance the formation of DSBs.
摘要
在标准环境温度和压力（SATP）下，在不同可控氧气水平下辐照的干燥和潮湿质粒DNA薄膜中，测量了由软X射线（1.5 keV）和光发射低能电子（0 - 30 eV）诱导的单链和双链断裂。从这些实验得出的值表明，在保持所有其他实验参数不变的情况下，羟基自由基（HO）的存在以及将气氛从氮气（N）改为氧气（O），对于X射线和低能电子，分别使双链断裂的形成增加了4.5倍和11.8倍。在潮湿DNA薄膜的氧化环境中，水和氧气结合导致的额外低能电子诱导损伤表现出超加性效应，这导致形成双链断裂的 值几乎比X射线光子获得的值高7倍。这些结果表明，氧气（O）、羟基自由基（HO）和低能电子有效地协同作用，增强了双链断裂的形成。
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