Peng Xingzhou, Wang Junjie, Deng Zihan, Wei Jianshuang, Xie Changqiang, Wang Yan, Han Jianlei, Chen Zhengyu, Du Jianghai, Zhang Zhihong
State Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Sanya, Hainan, 572025, China.
Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
Mater Today Bio. 2024 Aug 24;28:101209. doi: 10.1016/j.mtbio.2024.101209. eCollection 2024 Oct.
The development of novel phototheranostic agents with significant potential in bioimaging-guided therapy is highly desirable for precise tumor therapy. Herein, NIR laser-activated ruthenium phthalocyanine (PcRu) loaded sub-30 nm targeting lipid nanoparticles (α-PcRu-NPs) were fabricated for photoacoustic imaging (PAI)-guided photothermal therapy (PTT). Due to the formation of -type aggregation of PcRu in the core of the nanostructure, the α-PcRu-NPs exhibited high stability, efficient NIR absorption, reduced singlet oxygen generation, high photothermal activity, and intense photoacoustic signal. With the M2 macrophage target peptide (M2pep) modification and small size of α-PcRu-NPs, evaluations reveal that α-PcRu-NPs can specifically target and deeply penetrate the tumor foci. Under a high contrast PAI guidance with α-PcRu-NPs (744 nm, 0.35 μW), it also realizes superior photothermal therapy (PTT) for breast cancer under 670 nm laser irradiation (0.5 W/cm). The prominent therapeutic efficacy of α-PcRu-NP-based PTT not only directly kills tumor cells, but also enhances the immune response by promoting dendritic cell maturation and increasing cytotoxic T cell infiltration. Thus, this work broadens the applications of phthalocyanine derivatives as phototheranostics in the PAI-guided PTT field.
开发在生物成像引导治疗中具有巨大潜力的新型光诊疗剂对于精确肿瘤治疗非常必要。在此，制备了负载近红外（NIR）激光激活的酞菁钌（PcRu）的亚30纳米靶向脂质纳米颗粒（α-PcRu-NPs）用于光声成像（PAI）引导的光热治疗（PTT）。由于PcRu在纳米结构核心中形成了 - 型聚集体，α-PcRu-NPs表现出高稳定性、高效的近红外吸收、减少的单线态氧生成、高光热活性和强烈的光声信号。通过M2巨噬细胞靶向肽（M2pep）修饰以及α-PcRu-NPs的小尺寸，评估显示α-PcRu-NPs能够特异性靶向并深入穿透肿瘤病灶。在α-PcRu-NPs（744 nm，0.35 μW）的高对比度PAI引导下，它还在670 nm激光照射（0.5 W/cm）下实现了对乳腺癌的卓越光热治疗（PTT）。基于α-PcRu-NP的PTT的显著治疗效果不仅直接杀死肿瘤细胞，还通过促进树突状细胞成熟和增加细胞毒性T细胞浸润来增强免疫反应。因此，这项工作拓宽了酞菁衍生物作为光诊疗剂在PAI引导的PTT领域的应用。
