Zhao Mei, Ma Ning, Qiu Feng, Tian Xiangrong, Zhang Yan, Tang Haifeng, Liu Xinyou
Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China; Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China.
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
Fitoterapia. 2014 Sep;97:234-40. doi: 10.1016/j.fitote.2014.06.015. Epub 2014 Jun 28.
Reinvestigation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of a new ursane-type triterpenoid saponin 1 and a new taraxerane-type saponin 2, four known saponins 3-6 first isolated from the species, together with seven saponins 7-13 reported in the previous papers. The structures of saponins 1-6 were elucidated by extensive spectroscopic analysis and chemical evidences. The ursane-type and taraxerane-type triterpenoid saponins were obtained from genus Clematis for the first time, and the aglycone of saponin 1, 3β,28-dihydroxy-18αH-ursan-20-en was first encountered. The cytotoxicity of all the saponins was evaluated against human glioblastoma U251MG cell lines. The monodesmosidic saponins 1, 2 and 4-8 exhibited cytotoxic activity against the cells with IC50 values ranging from 6.95 to 38.51 μM.
对威灵仙根的正丁醇提取物进行再研究，从中分离得到一种新的乌苏烷型三萜皂苷1和一种新的蒲公英赛烷型皂苷2、首次从该物种中分离得到的4种已知皂苷3 - 6，以及先前文献报道的7种皂苷7 - 13。通过广泛的光谱分析和化学证据阐明了皂苷1 - 6的结构。乌苏烷型和蒲公英赛烷型三萜皂苷首次从铁线莲属植物中获得，且皂苷1的苷元3β,28 - 二羟基 - 18αH - 乌苏 - 20 - 烯是首次发现。评估了所有皂苷对人胶质母细胞瘤U251MG细胞系的细胞毒性。单糖链皂苷1、2和4 - 8对细胞表现出细胞毒性活性，IC50值范围为6.95至38.51 μM。
