Liu Ming, Wang Genzhu, Xiao Lin, Xu Xuanli, Liu Xiaohui, Xu Pingxiang, Lin Xiukun
Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China.
Mar Drugs. 2014 Jun 27;12(7):3838-51. doi: 10.3390/md12073838.
Bis(2,3-dibromo-4,5-dihydroxybenzyl) ether (BDDE) is a bromophenol isolated from marine algae. Previous reports have shown that BDDE possesses cytotoxic and antibacterial activity. In the present study, we demonstrate that BDDE displays broad-spectrum antifungal activities, especially on Botrytis cinerea. BDDE inhibits the growth of B. cinerea cultured on a solid medium of potato dextrose agar (PDA) as well as on the potato dextrose broth (PDB) medium. Moreover, BDDE decreases the incidence of fruit decay and severity of strawberries infected with B. cinerea. Further studies have revealed that BDDE decreases the germination rate and inhibits the mycelial growth of B. cinerea. The inhibition mechanisms are related to the disruption of the cell membrane integrity in B. cinerea spores and newly formed germ tubes. This study also suggests that BDDE possibly interacts with DNA via intercalation and minor groove binding. The studies provide evidence that BDDE has potential application in the control of gray mold after fruit harvest and the compound could serve as a candidate or lead template for rational drug design and for the development of antifungal agents.
双（2,3-二溴-4,5-二羟基苄基）醚（BDDE）是一种从海藻中分离出来的溴酚。先前的报道表明BDDE具有细胞毒性和抗菌活性。在本研究中，我们证明BDDE具有广谱抗真菌活性，尤其是对灰葡萄孢菌。BDDE抑制在马铃薯葡萄糖琼脂（PDA）固体培养基以及马铃薯葡萄糖肉汤（PDB）培养基上培养的灰葡萄孢菌的生长。此外，BDDE降低了感染灰葡萄孢菌的草莓的果实腐烂发生率和严重程度。进一步的研究表明，BDDE降低了灰葡萄孢菌的萌发率并抑制其菌丝体生长。抑制机制与灰葡萄孢菌孢子和新形成的芽管中细胞膜完整性的破坏有关。本研究还表明，BDDE可能通过嵌入和小沟结合与DNA相互作用。这些研究提供了证据，证明BDDE在果实采后灰霉病防治中具有潜在应用，该化合物可作为合理药物设计和抗真菌剂开发的候选物或先导模板。
